State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, Zhejiang Province, China.
Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14492-7. doi: 10.1073/pnas.1306579110. Epub 2013 Aug 12.
The growing world population and shrinkage of arable land demand yield improvement of rice, one of the most important staple crops. To elucidate the genetic basis of yield and uncover its associated loci in rice, we resequenced the core recombinant inbred lines of Liang-You-Pei-Jiu, the widely cultivated super hybrid rice, and constructed a high-resolution linkage map. We detected 43 yield-associated quantitative trait loci, of which 20 are unique. Based on the high-density physical map, the genome sequences of paternal variety 93-11 and maternal cultivar PA64s of Liang-You-Pei-Jiu were significantly improved. The large recombinant inbred line population combined with plentiful high-quality single nucleotide polymorphisms and insertions/deletions between parental genomes allowed us to fine-map two quantitative trait loci, qSN8 and qSPB1, and to identify days to heading8 and lax panicle1 as candidate genes, respectively. The quantitative trait locus qSN8 was further confirmed to be days to heading8 by a complementation test. Our study provided an ideal platform for molecular breeding by targeting and dissecting yield-associated loci in rice.
世界人口的增长和可耕地的减少，要求水稻（最重要的主食作物之一）的产量得到提高。为了阐明水稻产量的遗传基础并揭示其相关基因座，我们对广泛种植的超级杂交稻两优培九的核心重组自交系进行了重测序，并构建了一个高分辨率的连锁图谱。我们检测到 43 个与产量相关的数量性状基因座，其中 20 个是独特的。基于高密度物理图谱，父本品种 93-11 和母本品种 PA64s 的基因组序列得到了显著改善。大型重组自交系群体结合丰富的高质量单核苷酸多态性和双亲基因组之间的插入/缺失，使我们能够对两个数量性状基因座 qSN8 和 qSPB1 进行精细定位，并分别将其候选基因鉴定为 heading8 和 lax panicle1。通过互补测验进一步证实了数量性状基因座 qSN8 就是 heading8。我们的研究为靶向和剖析水稻产量相关基因座的分子育种提供了一个理想的平台。
