Liu Zhong-Zhi, Kong Jing-Bo, Li Feng-Zhi, Ma Long-Le, Liu Shu-Qin, Wang Le-Xin
Department of Cardiology, Liaocheng People's Hospital of Shandong University, Liaocheng, Shandong, Peoples' Republic of China;
Exp Clin Cardiol. 2013 Spring;18(2):e99-e101.
To investigate the effect of ischemic postconditioning on the expression of matrix metalloproteinase (MMP)-2 during ischemia-reperfusion of myocardium in a rabbit model.
Thirty-six male New Zealand white rabbits were randomly divided into sham, ischemia-reperfusion and ischemic postconditioning groups. Myocardial ischemia-reperfusion was created by ligating the left anterior descending coronary artery for 30 min followed by 3 h of reperfusion. Myocardial infarction sizes were determined by dual staining with triphenyltetrazolium chloride and trypan blue. Plasma levels of MMP-2 were measured using ELISA. Myocardial MMP-2 messenger RNA was analyzed by reverse transcription polymerase chain reaction.
The mean (± SD) infarct size in the ischemic postconditioning group was significantly smaller compared with the ischemia-reperfusion group (37.1±3.8% versus 57.5±1.9%; P=0.02). The incidence of ventricular tachycardia in the ischemic postconditioning group was also lower than in the ischemia-reperfusion group (8.5% versus 75%; P=0.003). MMP-2 messenger RNA expression in the ischemic postconditioning group was significantly lower compared with the ischemia-reperfusion group (0.4944±0.0476 versus 0.6989±0.0694; P=0.02).
Ischemic postconditioning reduces myocardial ischemia-reperfusion injury, possibly by inhibiting the expression of MMP-2.
在兔模型中研究缺血后处理对心肌缺血再灌注期间基质金属蛋白酶（MMP）-2表达的影响。
将36只雄性新西兰白兔随机分为假手术组、缺血再灌注组和缺血后处理组。通过结扎左冠状动脉前降支30分钟，然后再灌注3小时来建立心肌缺血再灌注模型。采用氯化三苯基四氮唑和台盼蓝双重染色法测定心肌梗死面积。使用酶联免疫吸附测定法检测血浆中MMP-2的水平。通过逆转录聚合酶链反应分析心肌MMP-2信使核糖核酸。
与缺血再灌注组相比，缺血后处理组的平均（±标准差）梗死面积显著减小（37.1±3.8%对57.5±1.9%；P=0.02）。缺血后处理组室性心动过速的发生率也低于缺血再灌注组（8.5%对75%；P=0.003）。与缺血再灌注组相比，缺血后处理组中MMP-2信使核糖核酸的表达显著降低（0.4944±0.0476对0.6989±0.0694；P=0.02）。
缺血后处理可减轻心肌缺血再灌注损伤，可能是通过抑制MMP-2的表达来实现的。
