利用集成微流控芯片方便地定量检测甲醇浓度。
Convenient quantification of methanol concentration detection utilizing an integrated microfluidic chip.
机构信息
Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.
出版信息
Biomicrofluidics. 2012 Aug 13;6(3):34111. doi: 10.1063/1.4746246. Print 2012 Sep.
A rapid and simple technique is proposed for methanol concentration detection using a PMMA (Polymethyl-Methacrylate) microfluidic chip patterned using a commercially available CO2 laser scriber. In the proposed device, methanol and methanol oxidase (MOX) are injected into a three-dimensional circular chamber and are mixed via a vortex stirring effect. The mixture is heated to prompt the formation of formaldehyde and is flowed into a rectangular chamber, to which fuchsin-sulphurous acid is then added. Finally, the microchip is transferred to a UV spectrophotometer for methanol detection purposes. The experimental results show that a correlation coefficient of R(2) = 0.9940 is obtained when plotting the optical density against the methanol concentration for samples and an accuracy as high as 93.1% are compared with the determined by the high quality gas chromatography with concentrations in the range of 2 ∼ 100 ppm. The methanol concentrations of four commercial red wines are successfully detected using the developed device. Overall, the results show that the proposed device provides a rapid and accurate means of detecting the methanol concentration for a variety of applications in the alcoholic beverage inspection and control field.
提出了一种使用商用 CO2 激光划线机对 PMMA（聚甲基丙烯酸甲酯）微流控芯片进行图案化来检测甲醇浓度的快速简单技术。在提出的装置中，甲醇和甲醇氧化酶（MOX）被注入到三维圆形室中，并通过涡流搅拌作用混合。将混合物加热以促使甲醛形成，并将其流入矩形室中，然后向其中添加品红-亚硫酸溶液。最后，将微芯片转移到紫外分光光度计中以进行甲醇检测。实验结果表明，当绘制样品的吸光度与甲醇浓度之间的关系时，得到相关系数 R(2)=0.9940，与高质量气相色谱法相比，其精度高达 93.1%，浓度范围为 2∼100ppm。使用开发的设备成功检测了四种商业红葡萄酒的甲醇浓度。总体而言，结果表明该装置为酒精饮料检测和控制领域的各种应用提供了一种快速准确的甲醇浓度检测方法。
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