Wu W C, Kuo H F, Hsueh Y K, Wang L Y
Graduate Institute of Plant Pathology, National Chung Hsing University, Taichung, Taiwan.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1990 May;23(2):162-5.
A typical colony of each of strains XW17, XW45, XW47, XW82, XW86, XW118, and XW138 of Xanthomonas campestris pv. citri was streaked onto nutrient agar, GY agar, minimal agar, and semi-enriched minimal agar plates, respectively. The agar plates, after incubated at 30 degrees C for 2-4 days, were inverted and stored at 4 degrees C in darkness. All of the X. campestris pv. citri strains retained their viability for at least 26 weeks on the semi-enriched minimal agar plates; whereas, cultures of these bacterial strains survived for only 2-10 weeks on the nutrient agar, 2-5 weeks on the GY agar plates and 12-26 weeks on the minimal agar plates. The bacterial strains which survived after 22-26 weeks on the semi-enriched minimal agar and minimal agar plates, respectively, also retained their ability to produce typical colonies and virulence. Thus, this semi-enriched minimal agar plate method is a simple and efficient way for maintaining the X. campestris pv. citri strains for a relatively long period.
分别将柑橘溃疡病菌的XW17、XW45、XW47、XW82、XW86、XW118和XW138菌株的典型菌落划线接种到营养琼脂、GY琼脂、基本琼脂和半富集基本琼脂平板上。将琼脂平板在30℃下培养2 - 4天，然后倒置并在4℃黑暗条件下保存。所有柑橘溃疡病菌株在半富集基本琼脂平板上至少可存活26周；而这些细菌菌株的培养物在营养琼脂上仅存活2 - 10周，在GY琼脂平板上存活2 - 5周，在基本琼脂平板上存活12 - 26周。分别在半富集基本琼脂平板和基本琼脂平板上22 - 26周后存活的细菌菌株，也保留了产生典型菌落和毒力的能力。因此，这种半富集基本琼脂平板法是一种简单有效的长期保存柑橘溃疡病菌株的方法。
