鉴定、计数和评估小鼠嗜酸性粒细胞的实验方案。

Protocols for identifying, enumerating, and assessing mouse eosinophils.

作者信息

Dyer Kimberly D, Garcia-Crespo Katia E, Percopo Caroline M, Sturm Eva M, Rosenberg Helene F

机构信息

Inflammation Immunobiology Section, Laboratory of Allergic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2013;1032:59-77. doi: 10.1007/978-1-62703-496-8_5.

DOI:10.1007/978-1-62703-496-8_5

PMID:23943444

Abstract

Eosinophils are prominent in allergic diseases, and their effector functions are studied in numerous gene-deleted and transgenic mouse models. However, mouse eosinophils and human eosinophils are not structurally or functionally equivalent, and assays designed to evaluate the properties of human eosinophils may or may not be reliable or effective in experiments targeting their murine counterparts. In this chapter, we emphasize methods focused on detection, isolation, and functional assessment of eosinophils from mouse tissue and present a protocol that promotes the growth and differentiation of eosinophils from unselected mouse bone marrow progenitors. Overall, these protocols provide a scaffold on which the relative contributions of mouse eosinophils can be evaluated.

摘要

嗜酸性粒细胞在过敏性疾病中很突出，并且在众多基因敲除和转基因小鼠模型中对其效应功能进行了研究。然而，小鼠嗜酸性粒细胞和人类嗜酸性粒细胞在结构或功能上并不等同，旨在评估人类嗜酸性粒细胞特性的检测方法在针对小鼠嗜酸性粒细胞的实验中可能可靠也可能不可靠或无效。在本章中，我们重点介绍针对从小鼠组织中检测、分离和功能评估嗜酸性粒细胞的方法，并提出一种促进未分选的小鼠骨髓祖细胞嗜酸性粒细胞生长和分化的方案。总体而言，这些方案提供了一个框架，据此可以评估小鼠嗜酸性粒细胞的相对作用。