Reichman Hadar, Rozenberg Perri, Munitz Ariel
Department of Clinical Microbiology and Immunology, The Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Israel.
Curr Protoc Immunol. 2017 Nov 1;119:14.43.1-14.43.22. doi: 10.1002/cpim.35.
Eosinophils are bone marrow-derived cells that differentiate in the bone marrow and migrate into the peripheral blood primarily under the regulation of interleukin (IL)-5. Eosinophil levels in the blood are relatively low. However, under steady-state conditions and in settings of allergic inflammation, parasite infections, or even cancer, they migrate and mainly reside in mucosal tissues where they have key effector and immune-modulating functions. Functional studies using eosinophils are not simple, since these cells are terminally differentiated and rapidly die in vitro. Thus, establishing simple methods to characterize, obtain, and functionally assess eosinophil activities is important. In this unit, we describe methodology for identifying tissue eosinophils by flow cytometry. In addition, we provide detailed methods for isolating eosinophils and for differentiating them from bone marrow cells for further functional studies. © 2017 by John Wiley & Sons, Inc.
嗜酸性粒细胞是源自骨髓的细胞，在骨髓中分化，并主要在白细胞介素（IL）-5的调节下迁移到外周血中。血液中的嗜酸性粒细胞水平相对较低。然而，在稳态条件下以及在过敏性炎症、寄生虫感染甚至癌症的情况下，它们会迁移并主要驻留在黏膜组织中，在那里它们具有关键的效应和免疫调节功能。使用嗜酸性粒细胞进行功能研究并不简单，因为这些细胞是终末分化的，并且在体外会迅速死亡。因此，建立简单的方法来表征、获取和功能评估嗜酸性粒细胞的活性很重要。在本单元中，我们描述了通过流式细胞术鉴定组织嗜酸性粒细胞的方法。此外，我们提供了分离嗜酸性粒细胞以及将它们与骨髓细胞区分开来以进行进一步功能研究的详细方法。© 2017约翰威立国际出版公司
