Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2021;2241:37-47. doi: 10.1007/978-1-0716-1095-4_4.
Human eosinophilic leukocytes are found in peripheral blood and tissues at homeostasis and at elevated levels in atopic disorders. As inbred strains of mice (Mus musculus) are currently the models of choice for the study of disease mechanisms in vivo, a full understanding of mouse eosinophils is critical for interpretation of experimental findings. Toward this end, several years ago we presented a protocol for generating mouse eosinophils in tissue culture from unselected bone marrow progenitors (Dyer et al., J Immunol 181: 4004-4009, 2008). This method has been implemented widely and has proven to be effective for generating phenotypically normal eosinophils from numerous mouse strains and genotypes. Here we provide a detailed version of this protocol, along with suggestions and notes for its careful execution. We have also included several protocol variations and suggestions for improvements.
人嗜酸性粒细胞存在于外周血和组织中，在过敏状态下和在特应性疾病中水平升高。由于近交系小鼠（Mus musculus）目前是研究体内疾病机制的首选模型，因此充分了解小鼠嗜酸性粒细胞对于解释实验结果至关重要。为此，几年前我们提出了一种从未选择的骨髓祖细胞在组织培养中生成小鼠嗜酸性粒细胞的方案（Dyer 等人，J Immunol 181:4004-4009,2008）。该方法已被广泛实施，并已被证明可有效地从许多小鼠品系和基因型中生成表型正常的嗜酸性粒细胞。在这里，我们提供了该方案的详细版本，并提供了有关其谨慎执行的建议和注意事项。我们还包括了一些方案变化和改进建议。
