Spectroscopy and Imaging, Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena (Germany), Fax: (+49) (0)3641 206 399; Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena (Germany).
Chemphyschem. 2013 Oct 21;14(15):3600-5. doi: 10.1002/cphc.201300543. Epub 2013 Aug 13.
We developed a Raman-compatible chip for isolating microorganisms from complex media. The isolation of bacteria is achieved by using antibodies as capture molecules. Due to the very specific interaction with the targets, this approach is promising for isolation of bacteria even from complex matrices such as body fluids. Our choice of capture molecules also enabled the investigation of samples containing yet unidentified bacteria, as the antibodies can capture a large variety of bacteria based on their analogue cell wall surface structures. The capability of our system is demonstrated for a broad range of different Gram-positive and Gram-negative germs. Subsequent identification is done by recording Raman spectra of the bacteria. Further, it is shown that classification with chemometric methods is possible.
我们开发了一种拉曼兼容芯片，用于从复杂介质中分离微生物。细菌的分离是通过使用抗体作为捕获分子来实现的。由于与目标的非常特异性相互作用，这种方法有望从复杂基质（如体液）中分离细菌。我们选择的捕获分子还使我们能够研究含有尚未鉴定的细菌的样本，因为抗体可以根据其类似的细胞壁表面结构捕获大量不同的细菌。我们的系统的功能通过记录各种革兰氏阳性和革兰氏阴性细菌的拉曼光谱来证明。此外，还表明可以通过化学计量方法进行分类。
