Kell Arnold J, Stewart Gale, Ryan Shannon, Peytavi Regis, Boissinot Maurice, Huletsky Ann, Bergeron Michel G, Simard Benoit
Steacie Institute for Molecular Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario, Canada K1A 0R6.
ACS Nano. 2008 Sep 23;2(9):1777-88. doi: 10.1021/nn700183g.
A series of vancomycin-modified nanoparticles were developed and employed in magnetic confinement assays to isolate a variety of Gram-positive and Gram-negative bacteria from aqueous solution. We determined that the orientation/architecture of vancomycin on the surface of the nanoparticles and the overall surface coverage is critical in mediating fast and effective interactions between the nanoparticle and the pathogen cell wall surface and only one orientation/architecture in a series of modified nanoparticles leads to the efficient and reproducible capture of several important pathogenic bacteria. Interestingly, as the nanoparticles increase in diameter (from approximately 50 to 2800 nm), it is necessary to incorporate a long linker between the nanoparticle surface and the vancomycin moiety in order for the surface bound probe to efficiently confine Gram-positive bacteria. Finally, we also determined that the time required for efficient labeling and subsequent magnetic confinement significantly decreases as the size of the nanoparticle and the vancomycin surface coverage on the nanoparticle increases. As disease detection technologies transition to "lab-on-a-chip" based platforms it is necessary to develop strategies to effectively and inexpensively preconcentrate cells from large volume to volumes more amenable to these types of microfluidic devices. These small molecule-modified superparamagnetic nanoparticles can provide a means by which this can be accomplished.
我们开发了一系列万古霉素修饰的纳米颗粒，并将其用于磁约束分析，以从水溶液中分离各种革兰氏阳性菌和革兰氏阴性菌。我们确定，纳米颗粒表面万古霉素的取向/结构以及整体表面覆盖率对于介导纳米颗粒与病原体细胞壁表面之间快速有效的相互作用至关重要，并且在一系列修饰的纳米颗粒中只有一种取向/结构能够有效且可重复地捕获几种重要的致病细菌。有趣的是，随着纳米颗粒直径的增加（从约50纳米到2800纳米），有必要在纳米颗粒表面和万古霉素部分之间引入一个长连接子，以便表面结合的探针能够有效地约束革兰氏阳性菌。最后，我们还确定，随着纳米颗粒尺寸以及纳米颗粒上万古霉素表面覆盖率的增加，有效标记和随后磁约束所需的时间会显著减少。随着疾病检测技术向基于“芯片实验室”的平台转变，有必要开发策略，以有效且廉价地将大量细胞预浓缩到更适合这些类型微流控设备的体积。这些小分子修饰的超顺磁性纳米颗粒可以提供实现这一目标的手段。
