Rossi Gabriele, Giordano Alessia, Pezzia Francesca, Kjelgaard-Hansen Mads, Paltrinieri Saverio
Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
Vet Clin Pathol. 2013 Sep;42(3):329-41. doi: 10.1111/vcp.12073. Epub 2013 Aug 14.
Serum activity of paraoxonase (PON1) decreases during inflammation in many species. Little information is available on paraoxon-based tests and the possible role of PON1 in dogs.
The objectives of the study were to validate an automated paraoxon-based assay to measure PON1 activity in canine serum, to determine its stability under different storage conditions, to determine a reference interval (RI) in healthy dogs, and to assess whether PON1 is of comparable diagnostic value as C-reactive protein (CRP) and α2-globulins.
Intra-assay and inter-assay imprecision, linearity under dilution (LUD), interference, and storage artifacts were evaluated. A PON1 RI was determined for healthy dogs, and PON1 activity, sensitivity, and specificity were compared with CRP and α2-globulins.
Intra- and inter-assay CVs were below 1.6% and 7.8%, respectively. The LUD test fitted the linear model. PON1 activity measurements were increased after addition of hemolysates and lipids, and after storage for 12 hours at room temperature, 72 hours at 4°C, and 6 months at -20°C. PON1 activity and CRP or α2-globulins did not correlate well. PON1 activity decreased significantly only in dogs with very high CRP concentrations. In contrast to CRP and α2-globulins, PON1 activity was not significantly different between dogs with and without inflammation.
The automated paraoxon-based method to assess serum canine PON1 activity was accurate and precise, but it was influenced by hemolysis, lipemia, and standard storage conditions. In this study, contrarily to CRP and α2-globulins, PON1 activity did not provide diagnostic value as a negative acute phase protein in dogs.
在许多物种的炎症过程中，对氧磷酶（PON1）的血清活性会降低。关于基于对氧磷的检测方法以及PON1在犬类中的可能作用，目前所知甚少。
本研究的目的是验证一种基于对氧磷的自动化检测方法，以测量犬血清中的PON1活性，确定其在不同储存条件下的稳定性，确定健康犬的参考区间（RI），并评估PON1与C反应蛋白（CRP）和α2球蛋白是否具有可比的诊断价值。
评估了批内和批间不精密度、稀释线性度（LUD）、干扰和储存假象。确定了健康犬的PON1 RI，并将PON1活性、敏感性和特异性与CRP和α2球蛋白进行了比较。
批内和批间CV分别低于1.6%和7.8%。LUD测试符合线性模型。添加溶血产物和脂质后，以及在室温下储存12小时、4°C下储存72小时和-20°C下储存6个月后，PON1活性测量值增加。PON1活性与CRP或α2球蛋白的相关性不佳。仅在CRP浓度非常高的犬中，PON1活性显著降低。与CRP和α2球蛋白不同，有炎症和无炎症的犬之间PON1活性无显著差异。
基于对氧磷的自动化方法评估犬血清PON1活性准确且精确，但受溶血、脂血和标准储存条件的影响。在本研究中，与CRP和α2球蛋白相反，PON1活性作为犬的负急性期蛋白不具有诊断价值。
