Yang Xu, Wang Dihua, Liang Youde, Yin Huayi, Zhang Shuang, Jiang Tao, Wang Yining, Zhou Yi
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China.
J Biomed Mater Res A. 2014 Jul;102(7):2395-407. doi: 10.1002/jbm.a.34906. Epub 2013 Sep 12.
This research investigated osteogenic potencies of Farthing-Fray-Chen Titanium (FFcTi) implant with transitional porous-solid structure. The material characteristics, biomechanical property, osteogenic performances were assessed. FFcTi showed similar roughness as sand-blasted and acid etched titanium (SA), but was more hydrophilic than SA and machined commercial pure titanium (MA). Young's modulus of FFcTi implant in compressive tests was 15.8 ± 6.3 GPa, which was close to bone. In vitro observations manifested excellent spreading abilities of MC3T3-E1 cell on FFcTi and SA. Adhesion rates of MC3T3-E1 cells at 4 h gradually decreased on MA, SA, and FFcTi surfaces (MA > SA, p < 0.01; SA > FFcTi, p < 0.05), while cell proliferation ability on FFcTi was weaker than MA during 1-6 days (p < 0.01) and similar to MA and SA in day 11. ALP activity of cells on FFcTi at 14 day was higher than MA and lower than SA (p < 0.01). In a bone defect model of rabbits, BIC and bone volum ratio within 50 μm were significantly higher for FFcTi than MA (BIC, p < 0.01; BT0.05, p < 0.05) while bone volume ratio within 100 and 500 μm were of no differences. Micro CT analysis also showed similar results to the histomorphometric data. Thus, we conclude that FFcTi with melting sphere based multiporous structure has a hydrophilic, rough surface, and close modulus to bone. In vitro, its low proliferation and ALP activity promotion were similar to other micro scale roughed surface. In vivo test showed better osteogenesis ability when compared with MA at least in 2 weeks. Thus, this Farthing-Fray-Chen Titanium implant seems to hold considerable potential for bone implant applications.
本研究调查了具有过渡性多孔-固体结构的法辛-弗雷-陈钛（FFcTi）种植体的成骨潜能。对其材料特性、生物力学性能和成骨性能进行了评估。FFcTi显示出与喷砂和酸蚀钛（SA）相似的粗糙度，但比SA和加工商用纯钛（MA）更具亲水性。FFcTi种植体在压缩试验中的杨氏模量为15.8±6.3 GPa，与骨相近。体外观察表明，MC3T3-E1细胞在FFcTi和SA上具有出色的铺展能力。MC3T3-E1细胞在4小时时在MA、SA和FFcTi表面的黏附率逐渐降低（MA＞SA，p＜0.01；SA＞FFcTi，p＜0.05），而在1-6天内FFcTi上的细胞增殖能力比MA弱（p＜0.01），在第11天时与MA和SA相似。在第14天时，FFcTi上细胞的碱性磷酸酶（ALP）活性高于MA且低于SA（p＜0.01）。在兔骨缺损模型中，FFcTi的骨整合接触（BIC）和50μm内的骨体积比显著高于MA（BIC，p＜0.01；BT0.05，p＜0.05），而100和500μm内的骨体积比无差异。显微CT分析也显示出与组织形态计量学数据相似的结果。因此，我们得出结论，具有基于熔球的多孔隙结构的FFcTi具有亲水性、粗糙表面且模量与骨相近。在体外，其低增殖和促进ALP活性与其他微尺度粗糙表面相似。体内试验表明，至少在2周内，与MA相比，其具有更好的成骨能力。因此，这种法辛-弗雷-陈钛种植体在骨植入应用中似乎具有相当大的潜力。
