Leszczyszyn D J, Jankowski J A, Viveros O H, Diliberto E J, Near J A, Wightman R M
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
J Biol Chem. 1990 Sep 5;265(25):14736-7.
Nicotinic receptor-mediated secretion of catecholamines from individual cultured bovine adrenal medullary chromaffin cells was measured and characterized with a voltametric microelectrode placed adjacent to the cells. Nicotine-induced secretion is associated with a large increase in chemical spikes that is temporally resolved into the apparent secretion of discrete packets of attomole quantities of easily oxidized molecules. These data are consistent with direct chemical measurement of single exocytotic events.
使用置于单个培养的牛肾上腺髓质嗜铬细胞附近的伏安微电极，测量并表征了烟碱型受体介导的儿茶酚胺分泌。尼古丁诱导的分泌与化学尖峰的大幅增加有关，这些化学尖峰在时间上可解析为阿托摩尔量的易氧化分子离散包的明显分泌。这些数据与单个胞吐事件的直接化学测量结果一致。
