Zheng Li-Juan, Qin Kun-Ming, Cai Hao, Cao Gang, Cai Bao-Chang
Engineering Center for Chinese Medicine Processing under State Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1504-9.
Baizhu Shaoyao San is a famous traditional Chinese medicine formula, which is efficient to diarrhea clinically. However, there are very few reports for extraction process of Baizhu Shaoyao San at present. In this article, an orthogonal test was conducted, with 10 major components and extractum of Baizhu Shaoyao San as indexes, and data was processed by the compound weight method, in order to detect four factors, including the concentration of ethanol, the dosage of ethanol, the soaking and extracting times, and optimize the extraction process of Baizhu Shaoyao San. The results showed that the optimal extraction process was adding 12-fold ethanol of 70% concentration, soaking for 1 hours, and extracting for twice, 2 hours each time. Among the indexes, the extracting time made significant impact. The optimized method is stable and reliable, and can provide reference for further development and utilization of the formula.
白术芍药散是著名的中药方剂，临床治疗腹泻疗效显著。然而，目前关于白术芍药散提取工艺的报道很少。本文采用正交试验，以白术芍药散中10种主要成分及提取物为指标，用复合加权法处理数据，考察乙醇浓度、乙醇用量、浸泡及提取次数4个因素，优化白术芍药散的提取工艺。结果表明，最佳提取工艺为加入12倍量70%乙醇，浸泡1小时，提取2次，每次2小时。各指标中，提取时间影响显著。该优化方法稳定可靠，可为该方剂的进一步开发利用提供参考。
