Liu Hong-Tao, Cao Ming-Pan, Zhang Xin-Feng, Si Jin-Ping
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1560-3.
This study was aimed to establish the HPLC fingerprints of the genus Chimonanthus leaves and compare the constituents distribution among five Chimonanthus species . The analysis was conducted on a C15 column (4.6 mm x 250 mm, 5 microm) with the mobile phase containing acetonitrile-water in gradient program: acetonitrile (B), 0-20 min, 6%-20%; 20-30 min, 20%-25%; 30-40 min, 25%-45%; 40-50 min, 45%-80%; 50-80 min, 80%-85%; 80-90 min, 85%-100%; 90-110 min, 100%. Flow rate was 0.8 mL x min(-1) and detection wavelength was 228 nm. Column temperature was set at 30 degrees C. The HPLC fingerprints of the five Chimonanthus species have been established. Ch. praecox, Ch. nitens, Ch. salicifolius, Ch. Zhejiangensis and Ch. grammatus have significant difference in constituents distribution and contents. Five standard substances as common compounds were confirmed in chromatography fingerprints. The method can be used as quality evaluation and classicfication of the genus Chimonanthus.
本研究旨在建立蜡梅属植物叶片的高效液相色谱指纹图谱，并比较5种蜡梅属植物中成分的分布情况。分析在C15柱（4.6 mm×250 mm，5μm）上进行，流动相为乙腈 - 水，采用梯度洗脱程序：乙腈（B），0 - 20分钟，6% - 20%；20 - 30分钟，20% - 25%；30 - 40分钟，25% - 45%；40 - 50分钟，45% - 80%；50 - 80分钟，80% - 85%；80 - 90分钟，85% - 100%；90 - 110分钟，100%。流速为0.8 mL·min⁻¹，检测波长为228 nm。柱温设定为30℃。已建立5种蜡梅属植物的高效液相色谱指纹图谱。蜡梅、亮叶蜡梅、柳叶蜡梅、浙江蜡梅和突托蜡梅在成分分布和含量上有显著差异。在色谱指纹图谱中确认了5种标准物质作为共有化合物。该方法可用于蜡梅属植物的质量评价和分类。
