[灵芝三萜类成分的色谱指纹图谱]

[Chromatographic fingerprints of triterpenoid constituents of Ganoderma lucidum].

作者信息

Ding Ping, Qiu Jinying, Liang Yingjiao, Wang Huiling

机构信息

College of Chinese Materia Medica, Guangzhou University of Chinese Madicine, Guangzhou 510405, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Sep;34(18):2356-9.

PMID:20030088

Abstract

OBJECTIVE

To establish chromatographic fingerprints of triterpenoid compounds in ganoderma RP-HPLC for the quality control of ganoderma.

METHOD

HPLC fingerprints were established. The chromatographic conditions were as follows: the separation was performed on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.8% perchloric acid as the mobile phases. The flow rate was 0.9 mL x min(-1), the detection wavelength was 254 nm and the temperature of column was at ambient temperature. The common patterns of HPTLC and HPLC fingerprints were separately obtained through "Chromafinger" solution software, and quality assessments were analyzed by similarity.

RESULT

The HPLC fingerprint pattern of G. lucidum consists of 18 peaks, among them 6 peaks were identified by chemical reference substances.

CONCLUSION

This study provided theory and experimental data in order to comprehensively evaluate the quality of ganoderma.

摘要

目的

建立灵芝中三萜类化合物的RP-HPLC色谱指纹图谱，用于灵芝的质量控制。

方法

建立HPLC指纹图谱。色谱条件如下：采用Diamonsil C18柱（4.6 mm×250 mm，5μm）分离，以含0.8%高氯酸的乙腈-水为流动相进行梯度洗脱。流速为0.9 mL·min⁻¹，检测波长为254 nm，柱温为室温。通过“Chromafinger”溶液软件分别获得HPTLC和HPLC指纹图谱的共有模式，并通过相似度进行质量评价。