Pérez-Ramírez Ursula, López-Orive Jesús Javier, Arana Estanislao, Salmerón-Sánchez Manuel, Moratal David
a Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València , Valencia , Spain.
Comput Methods Biomech Biomed Engin. 2015;18(4):446-55. doi: 10.1080/10255842.2013.818663. Epub 2013 Aug 15.
Anisotropy is one of the most meaningful determinants of biomechanical behaviour. This study employs micro-computed tomography (μCT) and image techniques for analysing the anisotropy of regenerative medicine polymer scaffolds. For this purpose, three three-dimensional anisotropy evaluation image methods were used: ellipsoid of inertia (EI), mean intercept length (MIL) and tensor scale (t-scale). These were applied to three patterns (a sphere, a cube and a right prism) and to two polymer scaffold topologies (cylindrical orthogonal pore mesh and spherical pores). For the patterns, the three methods provided good results. Regarding the scaffolds, EI mistook both topologies (0.0158, [-0.5683; 0.6001]; mean difference and 95% confidence interval), and MIL showed no significant differences (0.3509, [0.0656; 0.6362]). T-scale is the preferable method because it gave the best capability (0.3441, [0.1779; 0.5102]) to differentiate both topologies. This methodology results in the development of non-destructive tools to engineer biomimetic scaffolds, incorporating anisotropy as a fundamental property to be mimicked from the original tissue and permitting its assessment by means of μCT image analysis.
各向异性是生物力学行为最有意义的决定因素之一。本研究采用微计算机断层扫描（μCT）和图像技术来分析再生医学聚合物支架的各向异性。为此，使用了三种三维各向异性评估图像方法：惯性椭球体（EI）、平均截距长度（MIL）和张量尺度（t尺度）。这些方法应用于三种图案（一个球体、一个立方体和一个直角棱柱）以及两种聚合物支架拓扑结构（圆柱形正交孔网和球形孔）。对于这些图案，这三种方法都取得了良好的结果。对于支架，EI对两种拓扑结构都出现了误判（0.0158，[-0.5683；0.6001]；平均差异和95%置信区间），而MIL没有显示出显著差异（0.3509，[0.0656；0.6362]）。T尺度是更优的方法，因为它具有区分两种拓扑结构的最佳能力（0.3441，[0.1779；0.5102]）。这种方法导致了用于设计仿生支架的无损工具的开发，将各向异性作为要从原始组织中模拟的基本属性，并允许通过μCT图像分析对其进行评估。
