Xue Liang, Wu Qingping, Dong Ruimin, Kou Xiaoxia, Li Yonglai, Zhang Jumei, Guo Weipeng
1 School of Bioscience and Bioengineering, South China University of Technology , Guangzhou, Guangdong, China .
Foodborne Pathog Dis. 2013 Oct;10(10):888-95. doi: 10.1089/fpd.2013.1521. Epub 2013 Aug 15.
Noroviruses are regarded as the major causes of acute gastroenteritis worldwide, but their prevalence in sporadic diarrhea in South China remains unclear. This study was performed to characterize the genotypes of circulating norovirus strains associated with sporadic diarrhea cases in Guangzhou from November 2010 to January 2011. Among fecal specimens collected from 89 patients with acute diarrhea, nine samples (10.11%) were norovirus positive and 32 samples (35.96%) were rotavirus positive. The partial polymerase and the capsid regions of these norovirus samples were sequenced and phylogenetically analyzed. Three genotypes (GII.4, GII.6, and GII.b/GII.3) were identified, among which GII.4-2006b was the most predominant genotype (4/9, 44.4%), followed by GII.6 (3/9, 33.3%). A novel GII.4-2010 variant was first detected in China. Furthermore, the near full-length genome of the GZ2010-L26 strain, which belonged to GII.4-2006b, was sequenced and analyzed. Thus, the results of this study suggested that, second to rotavirus, noroviruses are the important pathogens responsible for sporadic acute gastroenteritis during winter in Guangzhou, and the GII.4-2006b variant remains the predominant genotype.
诺如病毒被认为是全球急性肠胃炎的主要病因，但在中国南方散发性腹泻中的流行情况仍不清楚。本研究旨在对2010年11月至2011年1月广州散发性腹泻病例中流行的诺如病毒株的基因型进行特征分析。在从89例急性腹泻患者采集的粪便标本中，9份样本（10.11%）诺如病毒呈阳性，32份样本（35.96%）轮状病毒呈阳性。对这些诺如病毒样本的部分聚合酶和衣壳区域进行测序并进行系统发育分析。鉴定出三种基因型（GII.4、GII.6和GII.b/GII.3），其中GII.4-2006b是最主要的基因型（4/9，44.4%），其次是GII.6（3/9，33.3%）。在中国首次检测到一种新型的GII.4-2010变异株。此外，对属于GII.4-2006b的GZ2010-L26株的近全长基因组进行了测序和分析。因此，本研究结果表明，诺如病毒是广州冬季仅次于轮状病毒的散发性急性肠胃炎的重要病原体，且GII.4-2006b变异株仍然是主要基因型。
