Colpi G M, Sagone P, Tognetti A, Campana A, Piffaretti-Yanez A, Balerna M
Andrological Surgery Department, 'Valduce' Hospital, Como, Italy.
Hum Reprod. 1990 Jul;5(5):600-5. doi: 10.1093/oxfordjournals.humrep.a137152.
The relationship and degree of association between the percentage of sperm swelling (HOS-test) and conventional semen variables was investigated in 263 consecutive ejaculates. The semen samples were exclusively obtained from men suspected of primary infertility. It was found that the correlation coefficients (Spearman's rho) followed the order: percentage of progressive motility at 3 h greater than count/ml greater than percentage of total motility at 3 h greater than percentage of normal spermatozoa. Of the three morphology sub-classes considered (sperm head, mid-piece and tail abnormalities), only mid-piece abnormalities correlated with the outcome of the HOS-test (rho = -0.409). Linear relationships between HOS-test results and sperm motility and morphology, but not sperm count, were indicated by LOWESS-smoothing. However, a linear relationship between the HOS-test, sperm count and a 'functional index' combining the conventional semen variables could be demonstrated after normalization of the data. Our findings suggest that the HOS-test may be of value in assessing the functional integrity and viability of spermatozoa; however, its prognostic power for fertility is probably not different from that of conventional semen variables.
在263份连续的射精样本中，研究了精子肿胀百分比（低渗肿胀试验）与传统精液参数之间的关系及关联程度。精液样本仅取自疑似原发性不育的男性。结果发现，相关系数（斯皮尔曼等级相关系数）排序如下：3小时时的前向运动精子百分比＞每毫升精子计数＞3小时时的总运动精子百分比＞正常精子百分比。在所考虑的三种形态亚类（精子头部、中段和尾部异常）中，只有中段异常与低渗肿胀试验结果相关（rho = -0.409）。局部加权散点平滑法（LOWESS）显示低渗肿胀试验结果与精子活力和形态之间存在线性关系，但与精子计数无关。然而，数据标准化后，可证明低渗肿胀试验、精子计数与结合传统精液参数的“功能指数”之间存在线性关系。我们的研究结果表明，低渗肿胀试验在评估精子的功能完整性和活力方面可能具有价值；然而，其对生育能力的预测能力可能与传统精液参数并无差异。
