Ramirez J P, Carreras A, Mendoza C
Departamento de Bioquímica y Biologia Molecular, Facultad de Ciencias, Universidad de Granada, Spain.
Andrologia. 1992 May-Jun;24(3):141-4. doi: 10.1111/j.1439-0272.1992.tb02627.x.
Sperm plasma membrane characteristics were analysed by a combined method, the HOS-eosine test (HOS-E test), that consists of the hypoosmotic swelling test (HOS test), and the eosine-Y staining. Semen samples were categorized into four groups (Normo-, Oligo-, Astheno-, and Oligoasthenozoospermic) and subjected to the standard analysis (spermiogram), HOS test, eosine-nigrosine test (reflecting sperm viability); and HOS-E test. HOS-E test makes it possible to distinguish four groups of spermatozoa: type 1, HOS+/eosine-; type 2, HOS-/eosine-; type 3, HOS-/eosine+; and type 4, HOS+/eosine+. Normozoospermic samples showed 61.2 +/- 1.4% type 1, 9.2 +/- 0.8% type 2, 22.6 +/- 1.1% type 3, and 6.8 +/- 0.6% type 4 spermatozoa. Oligozoospermic samples showed no significant differences in these values, whereas asthenozoospermic samples showed a higher percentage of types 3 and 4 and a lower percentage of type 1. Oligoasthenozoospermic samples showed high percentages of types 2, 3, and 4 and a low percentage of type 1. Sperm plasma membrane integrity is a necessary condition for motility and fertilization. So, it is not surprising that semen samples with abnormal motility showed a HOS-E result indicative of a defective plasma membrane.
采用一种联合方法——低渗肿胀-伊红试验（HOS-E试验）对精子质膜特征进行分析，该试验包括低渗肿胀试验（HOS试验）和伊红-Y染色。精液样本分为四组（正常精子症、少精子症、弱精子症和少弱精子症），并进行标准分析（精子图）、HOS试验、伊红-黑色素试验（反映精子活力）以及HOS-E试验。HOS-E试验能够区分出四类精子：1型，HOS+/伊红-；2型，HOS-/伊红-；3型，HOS-/伊红+；4型，HOS+/伊红+。正常精子症样本中1型精子占61.2±1.4%，2型占9.2±0.8%，3型占22.6±1.1%，4型占6.8±0.6%。少精子症样本在这些数值上无显著差异，而弱精子症样本中3型和4型精子的比例较高，1型精子的比例较低。少弱精子症样本中2型、3型和4型精子的比例较高，1型精子的比例较低。精子质膜完整性是精子运动和受精的必要条件。因此，运动异常的精液样本出现表明质膜有缺陷的HOS-E结果也就不足为奇了。
