Smith R, Madariaga M, Bustos-Obregon E
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
Int J Androl. 1992 Feb;15(1):5-13. doi: 10.1111/j.1365-2605.1992.tb01109.x.
The hypo-osmotic swelling (HOS) test has been proposed as a useful assay for evaluation of the functional competence of the human sperm membranes. To assess this further, the HOS-test was evaluated in 187 semen samples collected from fertile men and from male patients consulting for infertility. These samples were classified as normal, oligo-, astheno- or oligoasthenozoospermic on the basis of their standard semen variables. The percentage of total sperm tail swelling and of sperm exhibiting different tail swelling patterns was recorded. In the fertile men and in the group of patients with normal semen variables, significantly more (P less than 0.001) HOS-reactive sperm were observed after hypo-osmotic treatment in comparison with those groups exhibiting abnormal semen parameters. Swelling of the sperm in a hypo-osmotic medium was highly correlated with both progressive motility (r = 0.62, P less than 0.001) and sperm viability (r = 0.65, P less than 0.001). A weak positive correlation was also observed between sperm swelling and sperm morphological features (r = 0.31, P less than 0.005) and between sperm swelling and sperm concentration (r = 0.31, P less than 0.005). No significant correlation was observed between sperm swelling and in-vitro sperm fertilizing capacity as assessed by the zona-free hamster oocyte penetration assay. However the majority of the semen samples (87.3%) showing a normal penetration rate (greater than or equal to 10%) also exhibited a 60% (or higher) reaction in the HOS-test.(ABSTRACT TRUNCATED AT 250 WORDS)
低渗肿胀（HOS）试验已被提议作为评估人类精子膜功能能力的一种有用检测方法。为进一步评估此方法，对从有生育能力的男性以及因不育前来咨询的男性患者收集的187份精液样本进行了HOS试验评估。根据标准精液变量，将这些样本分为正常、少精子症、弱精子症或少弱精子症。记录了精子尾部总肿胀的百分比以及呈现不同尾部肿胀模式的精子百分比。与精液参数异常的组相比，在有生育能力的男性以及精液变量正常的患者组中，低渗处理后观察到的HOS反应性精子明显更多（P<0.001）。精子在低渗介质中的肿胀与进行性运动能力（r = 0.62，P<0.001）和精子活力（r = 0.65，P<0.001）均高度相关。在精子肿胀与精子形态特征（r = 0.31，P<0.005）以及精子肿胀与精子浓度（r = 0.31，P<0.005）之间也观察到弱正相关。通过无透明带仓鼠卵穿透试验评估，未观察到精子肿胀与体外精子受精能力之间存在显著相关性。然而，大多数（87.3%）显示正常穿透率（大于或等于10%）的精液样本在HOS试验中也表现出60%（或更高）的反应。（摘要截短于250字）
