Department of Chemical Engineering, Columbia, Missouri 65211, United States.
Biomacromolecules. 2013 Sep 9;14(9):3112-22. doi: 10.1021/bm4007369. Epub 2013 Aug 28.
Materials that are resistant to nonspecific protein adsorption are critical in the biomedical community. Specifically, nonfouling implantable biomaterials are necessary to reduce the undesirable, but natural foreign body response. The focus of this investigation is to demonstrate that polyampholyte hydrogels prepared with equimolar quantities of positively charged [2-(acryloyloxy)ethyl] trimethylammonium chloride (TMA) and negatively charged 2-carboxyethyl acrylate (CAA) monomers are a viable solution to this problem. TMA/CAA hydrogels were prepared and their physical and chemical properties were characterized. The fouling resistance of the TMA/CAA hydrogels were assessed at varying cross-linker densities using enzyme-linked immunosorbant assays (ELISAs). The results clearly demonstrate that TMA/CAA hydrogels are resistant to nonspecific protein adsorption. A unique advantage of the fouling resistant TMA/CAA system is that bioactive proteins can be covalently attached to these materials using standard conjugation chemistry. This was demonstrated in this study through a combination of ELISA investigations and short-term cell adhesion assays. The multifunctional properties of the TMA/CAA polyampholyte hydrogels shown in this work clearly demonstrate the potential for these materials for use as tissue regeneration scaffolds for many biomedical applications.
在生物医学领域，抗非特异性蛋白质吸附的材料至关重要。具体而言，为了减少不理想的但自然的异物反应，需要使用不易附着蛋白质的可植入生物材料。本研究的重点是证明，用等摩尔量的带正电荷的[2-（丙烯酰氧基）乙基]三甲基氯化铵（TMA）和带负电荷的 2-羧乙基丙烯酸酯（CAA）单体制备的聚两性电解质水凝胶是解决此问题的可行方法。制备了 TMA/CAA 水凝胶，并对其物理化学性质进行了表征。使用酶联免疫吸附测定（ELISA）评估了 TMA/CAA 水凝胶在不同交联密度下的抗污染能力。结果清楚地表明，TMA/CAA 水凝胶具有抗非特异性蛋白质吸附的能力。TMA/CAA 抗污染系统的一个独特优势是，可以使用标准的偶联化学将生物活性蛋白共价连接到这些材料上。本研究通过 ELISA 研究和短期细胞黏附试验相结合证明了这一点。本工作中展示的 TMA/CAA 聚两性电解质水凝胶的多功能特性清楚地表明了这些材料在许多生物医学应用中作为组织再生支架的潜力。
