Institute of Endemic Diseases, Xi'an Jiaotong University College of Medicine, Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an 710061, Shaanxi, PR China.
Osteoarthritis Cartilage. 2013 Nov;21(11):1781-9. doi: 10.1016/j.joca.2013.08.002. Epub 2013 Aug 12.
To clarify whether there is oxidative stress in Kashin-Beck disease (KBD) and if cartilage damage from reactive oxygen species (ROS) and oxidative stress mediate the chondral necrosis in articular cartilage of KBD.
We recruited 64 KBD patients, 46 healthy children from severely affected KBD regions, 81 healthy children from a non-severely affected KBD endemic regions, and 91 healthy control children from a non-KBD region. Ten patients with KBD from the non-severely affected KBD regions were included in the experiment. The 2,3-DAN fluorescence technique was used to test selenium in the hair and blood. The biochemical techniques used to test the indicators of oxidative stress included thiobarbituric acid reactive substances (TBARS) levels, and antioxidant enzyme activities in serum samples. Histochemical staining was used to detect proteoglycans in cartilage sections. The 4-hydroxy-2-nonenal (4-HNE) and 8-hydroxydeoxyguanisine (8-OHdG) were localized by immunohistochemistry.
The levels of TBARS in serum were significantly increased in KBD children. The levels of antioxidants in serum were significantly higher in both KBD and normal children from KBD regions than in the normal children from non-KBD regions. The percentage of chondrocytes staining for 4-HNE and 8-OHdG in KBD patients was significantly higher than in controls. Staining for 4-HNE and 8-OHdG in KBD patients was prominent in all zones of articular cartilage, especially in the necrotic chondrocytes of the deep zone.
KBD is an oxidative stress-related disease, and the oxidative stress in cartilage contributes to the pathology of cartilage damage in KBD.
阐明大骨节病（KBD）是否存在氧化应激，以及活性氧（ROS）和氧化应激引起的软骨损伤是否介导 KBD 关节软骨的坏死。
共纳入 64 例 KBD 患者、46 例来自严重 KBD 地区的健康儿童、81 例来自非严重 KBD 流行地区的健康儿童和 91 例来自非 KBD 地区的健康对照儿童。另外还纳入了 10 例来自非严重 KBD 流行地区的 KBD 患者。采用 2,3-DAN 荧光技术检测毛发和血液中的硒，使用生化技术检测血清中氧化应激的指标，包括硫代巴比妥酸反应物质（TBARS）水平和抗氧化酶活性。采用组织化学染色法检测软骨切片中的糖胺聚糖。采用免疫组织化学法检测 4-羟基-2-壬烯醛（4-HNE）和 8-羟基脱氧鸟苷（8-OHdG）的定位。
KBD 患儿血清 TBARS 水平显著升高。来自 KBD 地区的 KBD 和正常儿童血清中的抗氧化剂水平均显著高于非 KBD 地区的正常儿童。KBD 患者的软骨细胞中 4-HNE 和 8-OHdG 染色的百分比明显高于对照组。KBD 患者的 4-HNE 和 8-OHdG 染色在关节软骨的所有区域均明显，特别是在深层坏死的软骨细胞中。
KBD 是一种氧化应激相关疾病，软骨中的氧化应激导致了 KBD 中软骨损伤的病理变化。
