Freire Maria do Rosário Santos, Freitas Rafael, Colombo Fábio, Valença Alberto, Marques Aparecida Maria Cordeiro, Sarmento Viviane Almeida
Federal University of Bahia, Rua Araújo Pinho, 62 Canela, Salvador, Bahia, 40110-150, Brazil,
Clin Oral Investig. 2014 Apr;18(3):1005-13. doi: 10.1007/s00784-013-1058-4. Epub 2013 Aug 15.
This paper aims to evaluate the effects of laser (660 nm) and light-emitting diode (LED) (670 nm) irradiation in the cheek pouch mucosa of hamsters with oral mucositis (OM) induced by chemotherapy (Che) with 5-fluorouracil (5-FU).
In the preventive groups, the photobiomodulation was started 1 day before the drug administration and was performed every 48 h (Ia, IIa, Ib, and IIb). In the therapeutic groups (IIIa, IIIb, IVa, and IVb), the irradiations were started on the third day after the Che d(0) and was performed every 48 h. In both groups, animals were sacrificed 7 or 14 days after Che. In the positive control groups, the hamsters were subjected to Che but did not receive irradiation, and they were sacrificed in 7 days (Va) or 14 days (Vb). In the negative control groups, no procedures were done and the animals were sacrificed 7 days (Vc) or 14 days (Vd) after the experiment started.
The results indicated loss of body mass, xerostomia, and alopecia in the animals subjected to Che and the healing of OM to different degrees after the photobiomodulation treatment. Histologically, the positive control and experimental groups showed inflammation, predominately with lymphocytes and plasma cells, which tended to diminish with time. Epithelial atrophy, hyperemia, fibroblast proliferation, and vascular congestion were also observed at those intervals.
The best results were obtained from the preventive laser and LED photobiomodulation groups; both treatments were effective in diminishing the OM lesions.
A noninvasive and effective method with sparse side effects of OM would be desirable for use in cancer centers around the world.
本文旨在评估激光（660纳米）和发光二极管（LED）（670纳米）照射对接受5-氟尿嘧啶（5-FU）化疗（Che）诱导的口腔黏膜炎（OM）的仓鼠颊囊黏膜的影响。
在预防组中，光生物调节在给药前1天开始，每48小时进行一次（Ia、IIa、Ib和IIb）。在治疗组（IIIa、IIIb、IVa和IVb）中，照射在化疗第0天（d(0)）后的第三天开始，每48小时进行一次。在两组中，动物在化疗后7天或14天处死。在阳性对照组中，仓鼠接受化疗但未接受照射，并在7天（Va）或14天（Vb）处死。在阴性对照组中，不进行任何操作，动物在实验开始后7天（Vc）或14天（Vd）处死。
结果表明，接受化疗的动物出现体重减轻、口干和脱发，光生物调节治疗后口腔黏膜炎有不同程度的愈合。组织学上，阳性对照组和实验组均显示炎症，主要为淋巴细胞和浆细胞，炎症随时间有减轻趋势。在这些时间段还观察到上皮萎缩、充血、成纤维细胞增殖和血管充血。
预防性激光和LED光生物调节组取得了最佳效果；两种治疗方法均能有效减轻口腔黏膜炎病变。
一种无创且副作用少的有效口腔黏膜炎治疗方法将有望在世界各地的癌症中心使用。
