Mardani M, Afra S M, Tanideh N, Tadbir A Andisheh, Modarresi F, Koohi-Hosseinabadi O, Iraji A, Sepehrimanesh M
Department of Oral Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Students' Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Oral Dis. 2016 Jan;22(1):39-45. doi: 10.1111/odi.12375. Epub 2015 Dec 4.
Several studies have attempted to prevent or improve oral mucositis (OM) but have not produced a qualified treatment yet. This study evaluates the effects of Carum carvi L. (caraway) hydroalcoholic extract (CHE) as one of the traditional medicinal plants in 5-fluorouracil (5-FU)-induced OM in golden hamsters.
OM was induced in 54 male golden hamsters by 5-FU and cheek pouch scratching. Starting from day 12, 500 and 1000 mg kg(-1) per day topical CHE were administered. Pouch histopathology score, malondialdehyde and reduced glutathione contents, and activity of myeloperoxidase plus microbial cultures of cheek pouch, antimicrobial properties of CHE, and essential oil constituents were evaluated.
Lower histopathology score (0, 1, and 2) and malondialdehyde level, higher reduced glutathione level and activities of myeloperoxidase were detected in 1000 and 500 mg kg(-1) per day topical CHE and control groups, respectively (P < 0.001). The CHE was more potent against Staphylococcus epidermidis and Streptococcus intermedius. γ-Terpinene (37.2%) was identified as the main constituent of essential oil.
The use of CHE in topical form may be associated with reduced intensity of OM. This may be due to appropriate antibacterial activity and terpinene contents.
多项研究试图预防或改善口腔黏膜炎（OM），但尚未产生合格的治疗方法。本研究评估了作为传统药用植物之一的葛缕子水醇提取物（CHE）对5-氟尿嘧啶（5-FU）诱导的金黄仓鼠口腔黏膜炎的影响。
通过5-FU和颊囊刮擦在54只雄性金黄仓鼠中诱导口腔黏膜炎。从第12天开始，每天局部给予500和1000 mg kg⁻¹的CHE。评估颊囊组织病理学评分、丙二醛和还原型谷胱甘肽含量、髓过氧化物酶活性以及颊囊微生物培养、CHE的抗菌特性和精油成分。
在每天局部给予1000和500 mg kg⁻¹的CHE组及对照组中，分别检测到较低的组织病理学评分（0、1和2）和丙二醛水平，较高的还原型谷胱甘肽水平和髓过氧化物酶活性（P < 0.001）。CHE对表皮葡萄球菌和中间链球菌更有效。γ-萜品烯（37.2%）被鉴定为精油的主要成分。
局部使用CHE可能与降低口腔黏膜炎的严重程度有关。这可能归因于适当的抗菌活性和萜品烯含量。
