Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Cytotechnology. 2013 Dec;65(6):979-83. doi: 10.1007/s10616-013-9596-9. Epub 2013 Aug 15.
The current method for in vitro immunization (IVI) uses several antigens including toxins, food allergens, pathogenic bacteria, and self-antigen-derived peptides that induce an antigen-specific immune response in peripheral blood mononuclear cells (PBMCs). This protocol, however, requires donor blood collection and preparation of PBMCs before every IVI. In the present study, we aimed to design a more efficient system utilizing B cells immortalized with Epstein-Barr virus (EBV-B) as host cells for IVI to make antigen-specific antibodies. Results showed that previously antigen-sensitized, EBV-B cells exposed to the antigen along with IL-6, CpG oligonucleotides, and CD40 ligand signal produced antigen-specific antibodies. These results provide evidence for a novel and easy method to expand memory-type B cells and produce antigen-specific antibodies.
目前的体外免疫（IVI）方法使用了多种抗原，包括毒素、食物过敏原、致病菌和源自自身抗原的肽，这些抗原可在外周血单核细胞（PBMC）中诱导抗原特异性免疫反应。然而，该方案在每次 IVI 之前都需要采集供者血液并制备 PBMC。在本研究中，我们旨在设计一种更有效的系统，利用 EBV 转化的 B 细胞（EBV-B）作为宿主细胞进行 IVI，以产生抗原特异性抗体。结果表明，先前抗原致敏的 EBV-B 细胞在 IL-6、CpG 寡核苷酸和 CD40 配体信号的作用下暴露于抗原，可产生抗原特异性抗体。这些结果为扩展记忆型 B 细胞并产生抗原特异性抗体提供了一种新颖且简单的方法。
