Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Brazil.
Pharmacol Rep. 2013;65(3):600-13. doi: 10.1016/s1734-1140(13)71037-5.
Sulfated polysaccharides from red marine algae have presented a variety of potentially therapeutic biological effects, however, their antinocicpetive and anti-inflammatory properties are not well understood.
Male Swiss mice were pretreated with a sulfated polysaccharidic fraction obtained from the marine alga Acanthophora muscoides (AmII) (1, 3 or 9 mg/kg, iv) 30 min prior to either receiving an injection of 0.8% acetic acid or 1% formalin or prior to a thermal stimulus. AmII (1, 3 or 9 mg/kg, sc) was evaluated on carrageenan-, dextran- bradykinin-, histamine- and serotonin-induced rat paw edema models. AmII (500 μg, sc) was also injected into the paw. Additionally, mice were treated with the total sulfated polysaccharides from A. muscoides (Am-TSP) (20 mg/kg, ip) for 14 days.
AmII reduced the number of acetic acid-induced writhes and licking time in the second phase of the formalin test, but it did not alter the response latency in the hot plate test, suggesting that its antinociceptive action occurs through a peripheral mechanism. AmII did not reduce carrageenan-induced paw edema and MPO activity. However, it reduced dextran-, histamine- and serotonin-induced paw edemas, but not bradykinin-induced edema, suggesting that histamine is the major target of AmII anti-edematogenic activity. AmII injected into the paw did not evoke local edema. Furthermore, Am-TSP induced no consistent signs of systemic damage, as revealed by body mass, organs wet weight and by biochemical, hematological and histopathological analyses.
AmII has important antinociceptive and anti-inflammatory properties and represents an important therapeutic agent warranting future studies.
红海藻硫酸多糖具有多种潜在的治疗生物效应，但它们的抗伤害感受和抗炎特性尚未得到很好的理解。
雄性瑞士小鼠预先用从海洋藻类 Acanthophora muscoides 中获得的硫酸多糖（AmII）（1、3 或 9mg/kg，iv）预处理 30 分钟，然后接受 0.8%醋酸或 1%甲醛注射或热刺激。AmII（1、3 或 9mg/kg，sc）在角叉菜胶、葡聚糖、缓激肽、组胺和 5-羟色胺诱导的大鼠足肿胀模型中进行了评估。AmII（500μg，sc）也被注射到足中。此外，还向小鼠注射了 A. muscoides 的总硫酸多糖（Am-TSP）（20mg/kg，ip），共 14 天。
AmII 减少了乙酸诱导的扭体次数和福尔马林试验第二阶段的舔舐时间，但不改变热板试验的反应潜伏期，表明其镇痛作用通过外周机制发生。AmII 不减少角叉菜胶诱导的足肿胀和 MPO 活性。然而，它减少了葡聚糖、组胺和 5-羟色胺诱导的足肿胀，但不减少缓激肽诱导的足肿胀，表明组胺是 AmII 抗水肿活性的主要靶点。AmII 注入足中不会引起局部水肿。此外，Am-TSP 诱导的体重、器官湿重以及生化、血液学和组织病理学分析均未显示出一致的全身损伤迹象。
AmII 具有重要的镇痛和抗炎特性，是一种有前途的治疗药物。
