de Queiroz Ismael Nilo Lino, Quinderé Ana Luíza Gomes, Rodrigues José Ariévilo Gurgel, de Sousa Oliveira Vanderlei Edfranck, Ribeiro Natássia Albuquerque, da Conceição Rivanor Renata Line, Ribeiro Kátia Alves, Coura Chistiane Oliveira, Pereira Karuza Maria Alves, Chaves Hellíada Vasconcelos, Bezerra Mirna Marques, de Araújo Ianna Wivianne Fernandes, Benevides Norma Maria Barros
Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Department of Biochemistry and Molecular Biology, Federal University of Ceará, Bloco 907 Campus do Pici, Fortaleza, CEP: 60440-900, Brazil.
Inflamm Res. 2015 Dec;64(12):971-82. doi: 10.1007/s00011-015-0880-3. Epub 2015 Oct 1.
Wide biotechnological investigations of only a limited number of seaweed lectins have been performed. We previously demonstrated the anti-nociceptive and anti-inflammatory effects of a lectin isolated from the green seaweed Caulerpa cupressoides var. lycopodium (CcL). Herein, we further studied the mechanisms of action of CcL.
Classical acute inflammation models induced by different flogistic agents were used to evaluate the anti-inflammatory action of CcL. CcL was injected locally into the rat paw to verify a possible pro-inflammatory outcome.
CcL (0.1, 1 or 10 mg/kg; i.v.) reduced the carrageenan-induced rat paw edema and neutrophilic infiltration, which was not altered by either mucin (inhibitor of CcL carbohydrate-binding site) or ZnPP-IX (specific HO-1 inhibitor). Immunohistochemical analyses showed that CcL (1 mg/kg) reduced the expression of the cytokines IL-1β, TNF-α, IL-6 and COX-2. CcL (0.1, 1 or 10 mg/kg) inhibited dextran, and CcL (1 mg/kg) inhibited histamine-induced rat paw edema. Both effects were reversed by mucin inhibition. CcL (1 mg/kg) was ineffective for the treatment of serotonin- and bradykinin-induced rat paw edema. When injected via the i.pl. route, CcL (10 mg/kg) elicited rat paw edema involving a wide range of mediators.
The anti-inflammatory action of CcL involves the inhibition of IL-1β, TNF-α, IL-6 and COX-2 expression and histamine H1 receptors. When locally administered, CcL exerts pro-inflammatory actions.
仅对少数海藻凝集素进行了广泛的生物技术研究。我们之前证明了从绿藻羽藻（CcL）中分离出的一种凝集素具有抗伤害感受和抗炎作用。在此，我们进一步研究了CcL的作用机制。
使用由不同促炎剂诱导的经典急性炎症模型来评估CcL的抗炎作用。将CcL局部注射到大鼠爪中以验证可能的促炎结果。
CcL（0.1、1或10mg/kg；静脉注射）减轻了角叉菜胶诱导的大鼠爪肿胀和中性粒细胞浸润，粘蛋白（CcL碳水化合物结合位点抑制剂）或ZnPP-IX（特异性HO-1抑制剂）均未改变这种作用。免疫组织化学分析表明，CcL（1mg/kg）降低了细胞因子IL-1β、TNF-α、IL-6和COX-2的表达。CcL（0.1、1或10mg/kg）抑制了右旋糖酐，CcL（1mg/kg）抑制了组胺诱导的大鼠爪肿胀。两种作用均因粘蛋白抑制而逆转。CcL（1mg/kg）对治疗血清素和缓激肽诱导的大鼠爪肿胀无效。当通过腹腔注射途径给药时，CcL（10mg/kg）引起大鼠爪肿胀，涉及多种介质。
CcL的抗炎作用涉及抑制IL-1β、TNF-α、IL-6和COX-2的表达以及组胺H1受体。当局部给药时，CcL发挥促炎作用。
