Hong Bo-Young, Lee Tae-Kwon, Lim Sang-Min, Chang Seok Woo, Park Joonhong, Han Seung Hyun, Zhu Qiang, Safavi Kamran E, Fouad Ashraf F, Kum Kee Yeon
Division of Periodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA.
J Endod. 2013 Sep;39(9):1136-40. doi: 10.1016/j.joen.2013.05.001.
The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections.
Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted.
Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05).
The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.
本研究旨在通过GS-FLX Titanium焦磷酸测序技术，调查与无症状慢性根尖周炎相关的原发性和持续性牙髓感染中根管内微生物群的细菌群落概况。无效假设是原发性感染和持续性感染之间总体细菌群落概况的多样性没有差异。
对10个未经治疗的样本和8个根管充填样本进行焦磷酸测序分析。
对18个样本的分析共产生了124,767条16S rRNA基因序列（每个样本平均有6932条读数），这些序列在分类学上被归入803个操作分类单元（区分度为3%）、148个属和10个门，包括未分类的。拟杆菌门是原发性感染和持续性感染中最丰富的门。两个感染组之间的细菌多样性没有显著差异（P > 0.05）。基于树状图的细菌群落概况表明，两种感染中的细菌种群在结构和组成上没有显著差异（P > 0.05）。
目前的焦磷酸测序研究表明，持续性感染与原发性感染具有同样多样的细菌群落。
