Division of Endodontics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
Division of Basic Sciences, Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
Int Endod J. 2023 Feb;56(2):278-288. doi: 10.1111/iej.13864. Epub 2022 Nov 22.
To evaluate the root canal microbiome composition in cases of primary and secondary apical periodontitis.
Thirty-nine samples from patients with primary root canal infections obtained before root canal treatment, and 40 samples obtained during root-end resection procedures from previously filled cases with apical periodontitis were evaluated using 16S rRNA next-generation sequencing analysis (NGS). Demographic and clinical factors included age, sex, infection type, percussion sensitivity, and presence of pain. Differences in abundances of genera were evaluated using Kruskal-Wallis test. Alpha and beta diversity indices were calculated using mothur. The Shannon and Chao1 indices were used to measure alpha diversity. The Bray-Curtis dissimilarity was used to measure beta diversity. Differences in community composition were evaluated using analysis of similarity (ANOSIM) with Bonferroni correction for multiple comparisons.
Significantly fewer operational taxonomic units values were observed from samples from secondary infections (p < .0001). While no significant differences were observed in the Chao 1 index between primary and secondary infections, the Shannon alpha diversity was significantly lower in secondary relative to primary infections (p = .008). Among samples, sex, age (adult vs. older adult), percussion sensitivity, and presence of pain all showed no significant effects on community composition via an analysis of similarity (ANOSIM). However, community composition was significantly different depending on whether the sample was from a primary or secondary infection (R = .051, p = .03). Nine microbial genera comprised the predominant taxa observed among samples (>3.3%) and included Parvimonas, Fusobacterium, Campylobacter, Arachnia, Eubacterium, Prevotella, Peptostreptococcus, Fretibacterirum, and Pseudoramibacter. Significantly greater relative abundances of Prevotella, Peptostreptococcus, Veillonella, Lactucaseibacillus, and Dialister were observed in primary infections.
Primary endodontic infections are more diverse than secondary infections. The microbial composition is not associated with the clinical manifestations of apical periodontitis.
评估原发性和继发性根尖周炎病例的根管微生物组组成。
使用 16S rRNA 下一代测序分析（NGS）评估 39 例原发性根管感染患者在根管治疗前获得的样本和 40 例先前填充的根尖周炎患者在根管根尖切除术中获得的样本。人口统计学和临床因素包括年龄、性别、感染类型、叩诊敏感度和疼痛存在。使用 Kruskal-Wallis 检验评估属丰度的差异。使用 mothur 计算 alpha 和 beta 多样性指数。使用 Shannon 和 Chao1 指数衡量 alpha 多样性。使用 Bray-Curtis 不相似性衡量 beta 多样性。使用相似性分析（ANOSIM）评估群落组成的差异，并使用 Bonferroni 校正进行多次比较。
从继发性感染的样本中观察到的操作分类单位值明显较少（p < .0001）。虽然原发性和继发性感染之间的 Chao 1 指数没有显著差异，但继发性感染的 Shannon alpha 多样性明显低于原发性感染（p =.008）。在样本中，性别、年龄（成人与老年）、叩诊敏感度和疼痛存在均未通过相似性分析（ANOSIM）对群落组成产生显著影响。然而，根据样本是原发性还是继发性感染，群落组成存在显著差异（R =.051，p =.03）。9 种微生物属构成了样本中观察到的主要类群（>3.3%），包括 Parvimonas、Fusobacterium、Campylobacter、Arachnia、Eubacterium、Prevotella、Peptostreptococcus、Fretibacterirum 和 Pseudoramibacter。在原发性感染中，Prevotella、Peptostreptococcus、Veillonella、Lactucaseibacillus 和 Dialister 的相对丰度明显更高。
原发性牙髓感染比继发性感染更为多样化。微生物组成与根尖周炎的临床表现无关。
