College of Horticulture and Plant Protection and Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, PR China; Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Gene. 2013 Oct 15;529(1):1-6. doi: 10.1016/j.gene.2013.08.010. Epub 2013 Aug 13.
Liriomyza trifolii (Burgess), Liriomyza huidobrensis (Blanchard), and Liriomyza bryoniae (Kaltenbach), are three closely related and economically important leafminer pests in the world. This study examined the complete mitochondrial genomes of L. trifolii, L. huidobrensis and L. bryoniae, which were 16,141 bp, 16,236 bp and 16,183 bp in length, respectively. All of them displayed 37 typical animal mitochondrial genes and an A+T-rich region. The genomes were highly compact with only 60-68 bp of non-coding intergenic spacer. However, considerable differences in the A+T-rich region were detected among the three species. Results of this study also showed the two ribosomal RNA genes of the three species had very limited variable sites and thus should not provide much information in the study of population genetics of these species. Data generated from three leafminers' complete mitochondrial genomes should provide valuable information in studying phylogeny of Diptera, and developing genetic markers for species identification in leafminers.
三叶草斑潜蝇（Burgess）、褐斑潜蝇（Blanchard）和美洲斑潜蝇（Kaltenbach）是世界上三种密切相关且具有重要经济意义的潜叶蝇害虫。本研究测定了三叶草斑潜蝇、褐斑潜蝇和美洲斑潜蝇的完整线粒体基因组，它们的长度分别为 16141bp、16236bp 和 16183bp。它们都包含 37 个典型的动物线粒体基因和一个富含 A+T 的区域。这些基因组非常紧凑，非编码基因间隔区只有 60-68bp。然而，在这三个物种中，富含 A+T 的区域存在显著差异。本研究的结果还表明，三个物种的两个核糖体 RNA 基因具有非常有限的可变位点，因此在这些物种的种群遗传学研究中不应提供太多信息。从三种潜叶蝇的完整线粒体基因组中获得的数据应该为双翅目系统发育研究和潜叶蝇种间鉴定遗传标记的开发提供有价值的信息。
