Shen Xin, Meng Xueping, Tian Mei, Yan Binlun, Cheng Hanliang, Lu Wei, Chai Yuling
a Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science, Huaihai Institute of Technology , Lianyungang , China .
b Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology , Lianyungang , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3642-3. doi: 10.3109/19401736.2015.1079835. Epub 2015 Sep 2.
The complete mitochondrial genome of Coelomactra antiquata (Guangxi, in China, GX) was determined. It is 16 801 bp in length and is the first representative from this province. The mitochondrial genome encodes 35 genes, including 12 PCGs, two ribosomal RNA, and 21 transfer RNA genes. Atp8 and trnSer(UCN) genes are missing, compared with the typical gene content of animal mitochondrial genomes. Three (cob, nad1, nad4, and nad6) of the 12 PCGs in the mitochondrial genome initiate with the ATA, while other PCGs start with ATG. Two PCGs (atp6 and nad4L) end with incomplete stop codons (T-), and the remaining ones have complete stop codons (TAA or TAG). The largest non-coding region of the C. antiquata (GX) contains one section of tandem repeats (5 × 99 bp). Among all PCGs and rRNAs, the nad5 gene contains the maximum polymorphic sites (430), followed by nad4 (261) and cox2 (240). Two ribosomal RNA genes (srRNA and lrRNA) and cox1 are most conservative. The proportions of polymorphic sites in six genes (nad4, nad2, nad6, nad5, cox2, and nad3) are more than 20% (ranging from 20.25% to 25.21%). These high variable genes can be used as molecular markers in the population genetic analysis of the species.
测定了中国广西文蛤（GX）的完整线粒体基因组。其长度为16,801bp，是该省份的首个代表性样本。线粒体基因组编码35个基因，包括12个蛋白质编码基因（PCGs）、两个核糖体RNA基因和21个转运RNA基因。与动物线粒体基因组的典型基因组成相比，缺少Atp8和trnSer(UCN)基因。线粒体基因组中的12个PCGs中有3个（cob、nad1、nad4和nad6）以ATA起始，而其他PCGs以ATG起始。两个PCGs（atp6和nad4L）以不完全终止密码子（T-）结束，其余的具有完整终止密码子（TAA或TAG）。文蛤（GX）最大的非编码区包含一段串联重复序列（5×99bp）。在所有PCGs和rRNAs中，nad5基因的多态性位点最多（430个），其次是nad4（261个）和cox2（240个）。两个核糖体RNA基因（srRNA和lrRNA）和cox1最为保守。六个基因（nad4、nad2、nad6、nad5、cox2和nad3）的多态性位点比例超过20%（范围为20.25%至25.21%）。这些高变基因可作为该物种群体遗传分析的分子标记。
