Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, 110 016, India,
Appl Biochem Biotechnol. 2013 Nov;171(6):1351-61. doi: 10.1007/s12010-013-0432-7. Epub 2013 Aug 17.
Batch cultivation of Azadirachta indica hairy roots was carried out in different liquid-phase bioreactor configurations (stirred-tank, bubble column, bubble column with polypropylene basket, and polyurethane foam disc as root supports) to investigate possible scale-up of the A. indica hairy root culture for in vitro production of the biopesticide azadirachtin. The hairy roots failed to grow in the conventional bioreactor designs (stirred tank and bubble column). However, modified bubble column reactor (with polyurethane foam as root support) configuration facilitated high-density culture of A. indica hairy roots with a biomass production of 9.2 g l(-1)dry weight and azadirachtin yield of 3.2 mg g(-1) leading to a volumetric productivity of azadirachtin as 1.14 mg l(-1) day(-1). The antifeedant activity in the hairy roots was also evaluated by no choice feeding tests with known concentrations of the hairy root powder and its solvent extract separately on the desert locust Schistocerca gregaria. The hairy root powder and its solvent extract demonstrated a high level of antifeedant activity (with an antifeedant index of 97 % at a concentration of 2 % w/v and 83 % at a concentration of 0.05 % (w/v), respectively, in ethanol).
采用不同的液相生物反应器构型（搅拌釜、鼓泡塔、带聚丙烯篮的鼓泡塔和聚氨酯泡沫盘作为根支撑物）进行印楝毛状根的批量培养，以研究印楝毛状根培养的放大可能性，用于体外生产生物农药印楝素。毛状根在常规生物反应器设计（搅拌釜和鼓泡塔）中无法生长。然而，改良的鼓泡塔反应器（以聚氨酯泡沫作为根支撑物）的构型有利于印楝毛状根的高密度培养，生物量生产达到 9.2 g/L 干重，印楝素产量达到 3.2 mg/g，导致印楝素的比体积生产率为 1.14 mg/L·天。通过用已知浓度的毛状根粉末及其溶剂提取物分别对沙漠蝗 Schistocerca gregaria 进行无选择喂养试验，还评估了毛状根中的抗食活性。毛状根粉末及其溶剂提取物表现出高抗食活性（在浓度为 2%（w/v）时抗食指数为 97%，在浓度为 0.05%（w/v）时抗食指数为 83%，均在乙醇中）。
