School of Life Science, Liaoning University, Shenyang, 110036, China.
Appl Biochem Biotechnol. 2013 Nov;171(6):1444-53. doi: 10.1007/s12010-013-0441-6. Epub 2013 Aug 18.
A novel polysaccharide [Lentinus edodes mycelia polysaccharide 2 (LMP2)] with a molecular weight of 2.27 × 10(4) Da was isolated from the Lentinus edodes mycelia and purified by Sephadex G-200 and diethylaminoethyl-32 column chromatography. HPLC results indicated that LMP2 contained mannose, arabinose, galactose, xylose, and rhamnose with a relative molar ratio of 1:0.74:3.23:1.18:10.98. Its antitumor activity was evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and transwell assay. LMP2 showed a remarkable inhibitory effect on the proliferation of Hep-2 cells, and at the concentration of 200 mg/ml, the inhibition ratio was 37.2 % after 72 h. Furthermore, colony formation of Hep-2 cells was reduced significantly after treatment with LMP2. Invasion of Hep-2 cells was inhibited significantly by LMP2. These results suggested that LMP2 could be explored as a potential antitumor material for laryngeal carcinoma.
一种新型多糖[香菇菌丝体多糖 2（LMP2）]，分子量为 2.27×10(4)Da，从香菇菌丝体中分离出来，并通过 Sephadex G-200 和二乙氨基乙基-32 柱色谱进行纯化。HPLC 结果表明，LMP2 含有甘露糖、阿拉伯糖、半乳糖、木糖和鼠李糖，相对摩尔比为 1:0.74:3.23:1.18:10.98。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定法、集落形成测定法和 Transwell 测定法在体外评估了其抗肿瘤活性。LMP2 对 Hep-2 细胞的增殖表现出显著的抑制作用，在 200mg/ml 浓度下，72h 后抑制率为 37.2%。此外，LMP2 处理后 Hep-2 细胞的集落形成明显减少。LMP2 显著抑制 Hep-2 细胞的侵袭。这些结果表明，LMP2 可以作为喉癌潜在的抗肿瘤物质进行探索。
