Adaramoye Oluwatosin A, Arisekola Muritala
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan.
Niger J Physiol Sci. 2013 Jun 30;28(1):9-15.
In previous studies, we established that kolaviron (KV) (a biflavonoid from Garcinia kola seeds) elicited anti-oxidative and hepatoprotective effects in Wistar rats chronically treated with ethanol. The present study investigates the possible ameliorative effect of KV against ethanol-induced reproductive toxicity in male Wistar rats. Twenty-eight rats were randomly divided into four groups of seven animals each; Group 1 (control) was administered corn oil, group 2 was given 45%v/v ethanol at 3g/kg body weight, group 3 received ethanol and KV (200mg/kg) simultaneously and group 4 received KV alone. All drugs were given daily by oral gavage for 21 consecutive days. Ethanol treatment resulted in a significant (p<0.05) decrease in relative weight of testis of the animals. In the spermatozoa, ethanol intoxication resulted in 54%, 21% and 38% decreases in testicular protein content, sperm motility and count, respectively. In addition, ethanol administration enhanced lipid peroxidation (LPO) process assessed by the accumulation of malondialdehyde (MDA) in the testis. Precisely, MDA level was increased by 121% in the testis of ethanol-treated rats relative to the control. Furthermore, levels of testicular glutathione and activities of testicular antioxidant enzymes such as superoxide dismutase and catalase were significantly (p<0.05) reduced in ethanol-treated rats. Histopathology showed extensive degenerative changes in seminiferous tubules and defoliation of spermatocytes in testis of ethanol-treated rats. Interestingly, co-administration of KV with ethanol led to almost complete inhibition of testicular LPO thereby enhancing antioxidant status of the testis. Overall, KV ameliorates ethanol-induced toxic assault on testis and improves seminal qualities of the rats.
在先前的研究中，我们证实可乐维隆（KV）（一种来自可乐果种子的双黄酮）对长期用乙醇处理的Wistar大鼠具有抗氧化和肝脏保护作用。本研究调查了KV对乙醇诱导的雄性Wistar大鼠生殖毒性的可能改善作用。28只大鼠随机分为四组，每组7只动物；第1组（对照组）给予玉米油，第2组给予45%v/v乙醇，剂量为3g/kg体重，第3组同时给予乙醇和KV（200mg/kg），第4组单独给予KV。所有药物均通过口服灌胃每日给药，连续给药21天。乙醇处理导致动物睾丸相对重量显著（p<0.05）下降。在精子方面，乙醇中毒导致睾丸蛋白质含量、精子活力和数量分别下降54%、21%和38%。此外，乙醇给药增强了通过睾丸中丙二醛（MDA）积累评估的脂质过氧化（LPO）过程。确切地说，与对照组相比，乙醇处理大鼠睾丸中的MDA水平增加了121%。此外，乙醇处理大鼠睾丸中的谷胱甘肽水平以及睾丸抗氧化酶如超氧化物歧化酶和过氧化氢酶的活性显著（p<0.05）降低。组织病理学显示，乙醇处理大鼠睾丸的生精小管有广泛的退行性变化，精母细胞有脱叶现象。有趣的是，KV与乙醇共同给药几乎完全抑制了睾丸LPO，从而提高了睾丸的抗氧化状态。总体而言，KV改善了乙醇对睾丸的毒性攻击，并提高了大鼠的精液质量。
