Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Viruses. 2013 Aug 15;5(8):2005-18. doi: 10.3390/v5082005.
Caprine arthritis encephalitis virus (CAEV) is a lentivirus that infects both goats and sheep and is closely related to maedi-visna virus that infects sheep; collectively, these viruses are known as small ruminant lentiviruses (SRLV). Infection of goats and sheep with SRLV typically results in discrete inflammatory diseases which include arthritis, mastitis, pneumonia or encephalomyelitis. SRLV-infected animals concurrently demonstrating lentivirus-associated lesions in tissues of lung, mammary gland, joint synovium and the central nervous system are either very rare or have not been reported. Here we describe a novel CAEV promoter isolated from a sheep with multisystemic lentivirus-associated inflammatory disease including interstitial pneumonia, mastitis, polyarthritis and leukomyelitis. A single, novel SRLV promoter was cloned and sequenced from five different anatomical locations (brain stem, spinal cord, lung, mammary gland and carpal joint synovium), all of which demonstrated lesions characteristic of lentivirus associated inflammation. This SRLV promoter isolate was found to be closely related to CAEV promoters isolated from goats in northern California and other parts of the world. The promoter was denoted CAEV-ovine-MS (multisystemic disease); the stability of the transcription factor binding sites within the U3 promoter sequence are discussed.
山羊关节炎脑炎病毒（CAEV）是一种感染山羊和绵羊的慢病毒，与感染绵羊的梅迪-维斯纳病毒密切相关；这些病毒统称为小反刍动物慢病毒（SRLV）。SRLV 感染山羊和绵羊通常会导致关节炎、乳腺炎、肺炎或脑脊髓炎等离散性炎症性疾病。同时在肺部、乳腺、关节滑膜和中枢神经系统组织中表现出慢病毒相关病变的 SRLV 感染动物非常罕见或尚未报道。在这里，我们描述了一种从患有多系统慢病毒相关炎症性疾病（包括间质性肺炎、乳腺炎、多发性关节炎和白细胞脑脊髓炎）的绵羊中分离出的新型 CAEV 启动子。从五个不同的解剖部位（脑干、脊髓、肺、乳腺和腕关节滑膜）克隆和测序了一个单一的新型 SRLV 启动子，所有这些部位都表现出与慢病毒相关炎症的特征性病变。这种 SRLV 启动子分离株与加利福尼亚州北部和世界其他地区从山羊中分离出的 CAEV 启动子密切相关。该启动子被命名为 CAEV-绵羊-MS（多系统疾病）；讨论了 U3 启动子序列中转录因子结合位点的稳定性。
