Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
Nat Methods. 2013 Oct;10(10):981-4. doi: 10.1038/nmeth.2603. Epub 2013 Aug 18.
Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.
聚（ADP-核糖）化由一组称为 PARP 的酶催化。我们描述了一种鉴定人类天冬氨酸和谷氨酸 ADP-核糖基化蛋白质组的方法。我们在 340 种参与广泛核功能的蛋白质上鉴定了 1048 个 ADP-核糖基化位点；其中包括许多以前未知的 PARP 下游靶标，其 ADP-核糖基化对 PARP 抑制剂处理敏感。我们还证实，在完整细胞中，尼拉帕尼对 PARP 活性几乎没有影响。
