Zhou Qiwu, Lu Hao, He Ping, Yan Dujian, Zhao Baoyu, Zhang Liang, Wen Weili, Yang Xiaowen
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Wei Sheng Wu Xue Bao. 2013 May 4;53(5):478-85.
Endophytic fungi were detected and isolated from the stems, leaves, petioles and seeds of Oxytropis glabra DC sampled from Alashan of Inner Mongolia to investigate the infection rate and species distribution in different tissues.
The endophytic fungi infection rate and distribution of species in different tissues were investigated by making temporary slides, staining, isolation and identification.
Endophytic fungi were detected and observed from all parts (stems, leaves, petioles and seeds) of the plant by temporary slides staining. A total of 79 isolates were cultivated from 4 different tissues by common separation methods, which belonged to 10 genera after identification. The infection rate and separation rate were seeds > leaves > stems > petioles. Undifilum oxytropis, Embellisia sp. L12 and Fusarium equiseti were the dominant species in this plant with the relative isolate frequency 77.32%, 64.00% and 50.00%, respectively.
Endophytic fungi were commonly found in the all parts of Oxytropis glabra DC. There was an obviously difference in quantity, species and distribution of the endophytic fungi between different parts of plant. Seeds and leaves were the most vulnerable to infection and colonization by the endophytic fungi.
从采自内蒙古阿拉善的光棘豆的茎、叶、叶柄和种子中检测并分离内生真菌，以研究不同组织中的感染率和物种分布。
通过制作临时玻片、染色、分离和鉴定来研究不同组织中内生真菌的感染率和物种分布。
通过临时玻片染色从植物的所有部位（茎、叶、叶柄和种子）检测到并观察到内生真菌。通过常规分离方法从4种不同组织中共培养出79株分离物，鉴定后属于10个属。感染率和分离率为种子>叶>茎>叶柄。棘豆内生单孢菌、埃里砖格孢属L12和尖孢镰刀菌是该植物中的优势种，相对分离频率分别为77.32%、64.00%和50.00%。
光棘豆的所有部位普遍存在内生真菌。植物不同部位内生真菌的数量、种类和分布存在明显差异。种子和叶最易受到内生真菌的感染和定殖。
