Drenjancevic Ines, Kibel Aleksandar, Kibel Dijana, Seric Vatroslav, Cosic Anita
Department of Physiology and Immunology, School of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Croatia.
Undersea Hyperb Med. 2013 Jul-Aug;40(4):319-28.
Different protocols of hyperbaric oxygenation (HBO2) are used for research purposes; however, data on the changes in blood pressure, oxidative stress and acid-base and gas status induced by various oxygenation protocols are scarce and conflicting. The aim of this study was to examine the effects of an acute session of HBO2 [2 bar (200 kPa) for two hours] on arterial systolic and diastolic blood pressure, arterial blood gases and acid-base status, and oxidative stress in rats. Sprague-Dawley rats (12-15 weeks) were examined prior to, immediately and 24 hours after a two-hour HBO2 exposure at 2 bars. The femoral artery was cannulated to determine blood pressure, and blood samples were collected to measure blood gases and acid-base status, Ferric reducing antioxidant power ability of plasma (FRAP) and thiobarbituric acid reactive substances (TBARS). Immediately after HBO2 systolic and diastolic blood pressure significantly decreased (from 138 +/- 14/103 +/- 13 to 113 +/- 12/72 +/- 16 mmHg). However, these values were still inside the normal physiological range. pH decreased (from 7.34 +/- 0.05 to 7.28 +/- 0.05), pCO2 decreased (from 7.07 +/- 0.89 to 5.76 +/- 0.50 kPa), pO2 increased (from 12.48 +/- 0.88 to 13.68 +/- 2.4 kPa), plasma bicarbonate decreased (from 27.04 +/- 3.25 to 20.52 +/- 3.02 mmol/L). Exposure to HBO2 immediately increased TBARS levels (from 0.17 +/- 0.09 to 21.79 +/- 1.05 microM/MDA), while FRAP levels were not significantly changed. Measurements on separate animals 24 hours after a single HBO2 exposure showed no differences in comparison to control animals, except for pO2, which was significantly lower (11.10 +/- 0.31 kPa). The results define values of important parameters, serving as a necessary basis for complex analysis of HBO2 effects in research on rat animal models.
不同的高压氧疗（HBO2）方案用于研究目的；然而，关于各种氧疗方案引起的血压、氧化应激以及酸碱和气体状态变化的数据稀缺且相互矛盾。本研究的目的是检验急性HBO2疗程[2巴（200千帕），持续两小时]对大鼠动脉收缩压和舒张压、动脉血气和酸碱状态以及氧化应激的影响。在2巴的条件下对Sprague-Dawley大鼠（12 - 15周龄）进行两小时的HBO2暴露之前、暴露即刻以及暴露后24小时进行检查。通过插入股动脉来测定血压，并采集血样以测量血气和酸碱状态、血浆铁还原抗氧化能力（FRAP）以及硫代巴比妥酸反应性物质（TBARS）。HBO2暴露后即刻，收缩压和舒张压显著下降（从138±14/103±13降至113±12/72±16毫米汞柱）。然而，这些值仍在正常生理范围内。pH值下降（从7.34±0.05降至7.28±0.05），pCO2下降（从7.07±0.89降至5.76±0.50千帕），pO2升高（从12.48±0.88升至13.68±2.4千帕），血浆碳酸氢盐下降（从27.04±3.25降至20.52±3.02毫摩尔/升）。暴露于HBO2后即刻，TBARS水平升高（从0.17±0.09升至21.79±1.05微摩尔/丙二醛），而FRAP水平无显著变化。在单次HBO2暴露24小时后对单独的动物进行测量，与对照动物相比没有差异，除了pO2显著更低（11.10±0.31千帕）。这些结果确定了重要参数的值，为在大鼠动物模型研究中对HBO2效应进行综合分析提供了必要依据。
