School of Printing and Packaging, Wuhan Univ., Luo-jia-shan, Wuhan, Hubei Province 430079, China.
J Food Sci. 2013 Aug;78(8):N1301-6. doi: 10.1111/1750-3841.12208.
Garlic oil is considered as a natural broad-spectrum antibiotic because of its well-known antimicrobial activity. However, the characteristics of easy volatility and poor aqueous solubility limit the application of garlic oil in industry. The purpose of the present work is to develop and evaluate an oil-free microemulsion by loading garlic oil in microemulsion system. Microemulsions were prepared with ethoxylated hydrogenated castor (Cremophor RH40) as surfactant, n-butanol (or ethanol) as cosurfactant, oleic acid-containing garlic oil as oil phase, and ultrapure water as water phase. The effects of the ratio of surfactant to cosurfactant and different oil concentration on the area of oil-in-water (O/W) microemulsion region in pseudoternary phase diagrams were investigated. The particle size and garlic oil encapsulation efficiency of the formed microemulsions with different formulations were also investigated. In addition, the antimicrobial activity in vitro against Escherichia coli and Staphylococcus aureus was assessed. The experimental results show that a stable microemulsion region can be obtained when the mass ratio of surfactant to cosurfactant is, respectively, 1:1, 2:1, and 3:1. Especially, when the mixture surfactants of RH40/n-butanol 2/1 (w/w) is used in the microemulsion formulation, the area of O/W microemulsion region is 0.089 with the particle size 13.29 to 13.85 nm and garlic oil encapsulation efficiency 99.5%. The prepared microemulsion solution exhibits remarkable antibacterial activity against S. aureus.
大蒜油因其具有良好的抗菌活性而被认为是一种天然的广谱抗生素。然而，其易挥发性和较差的水溶性特点限制了大蒜油在工业中的应用。本工作旨在通过将大蒜油载入微乳体系中开发并评价一种不含油的微乳。采用以乙氧基化氢化蓖麻油（Cremophor RH40）为表面活性剂、正丁醇（或乙醇）为助表面活性剂、含油酸的大蒜油为油相、超纯水为水相的体系制备微乳。考察了表面活性剂与助表面活性剂的比例以及不同油浓度对伪三元相图中水包油（O/W）微乳区面积的影响。还考察了不同配方形成的微乳的粒径和大蒜油包封效率。此外，评估了其对大肠杆菌和金黄色葡萄球菌的体外抗菌活性。实验结果表明，当表面活性剂与助表面活性剂的质量比分别为 1:1、2:1 和 3:1 时，可以获得稳定的微乳区。特别是当微乳配方中使用 RH40/正丁醇 2/1（w/w）混合表面活性剂时，O/W 微乳区的面积为 0.089，粒径为 13.29 至 13.85nm，大蒜油包封效率为 99.5%。所制备的微乳溶液对金黄色葡萄球菌表现出显著的抗菌活性。
