随着年龄的增长，特异性 CD8 T 细胞反应受损并不是由于 TCR 转基因 T 细胞上 CD90 表达减少所致。

Impaired specific CD8 T cell response with aging is not due to decreased expression of CD90 on TCR transgenic T cells.

机构信息

Department of Biology, Drexel University, 3245 Chestnut Street, Philadelphia, PA 19104, USA.

出版信息

Immun Ageing. 2013 Aug 19;10(1):36. doi: 10.1186/1742-4933-10-36.

BACKGROUND

CD90 (Thy-1) is a small glycoprotein that is particularly abundant on the surface of mouse thymocytes and peripheral T cells, and is often used as a marker in adoptive transfer experiments to distinguish donor and recipient T cells with different CD90 subtypes. We have performed adoptive transfer experiments with T cell receptor transgenic (TCR Tg) mice to study the impaired CD8 T cell response with aging.

FINDINGS

After stimulation with a CD8 T cell epitope, HA518-524, the response of TCR Tg CD8 T cells from aged mice was decreased compared to the response of TCR Tg T cells from young mice. CD90 expression was also substantially decreased on the TCR Tg CD8 T cells of aged mice. However, the responses of CD90hi and CD90low CD8 T cells of the aged mice were similar in both early activation and proliferation, demonstrating that the impaired Tg T cell response with aging is not associated with the altered CD90 expression on CD8 T cells.

CONCLUSIONS

The impaired Tg CD8 T cell response in aged mice is not due to age-associated changes in CD90 expression on Tg CD8 T cells.

背景

CD90（Thy-1）是一种小的糖蛋白，在小鼠胸腺细胞和外周 T 细胞表面特别丰富，常用于过继转移实验中，以区分具有不同 CD90 亚型的供体和受体 T 细胞。我们使用 T 细胞受体转基因（TCR Tg）小鼠进行了过继转移实验，以研究衰老导致的 CD8 T 细胞应答受损的情况。

研究发现

与年轻 TCR Tg T 细胞相比，经 CD8 T 细胞表位 HA518-524 刺激后，老年 TCR Tg CD8 T 细胞的应答减少。老年 TCR Tg CD8 T 细胞上的 CD90 表达也显著降低。然而，在早期激活和增殖过程中，老年 TCR Tg CD8 T 细胞中 CD90hi 和 CD90low 亚群的应答相似，表明衰老导致的 Tg T 细胞应答受损与 CD8 T 细胞上 CD90 表达的改变无关。