Sun Tingting, Yu Chao, Gao Yuhua, Zhao Chenqiong, Hua Jinlian, Cai Lianshun, Guan Weijun, Ma Yuehui
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
Institute of Basic Medicine, Jiamusi University, Jiamusi 154007, People's Republic of China.
Biosci Rep. 2014 Apr 1;34(2). doi: 10.1042/BSR20130095.
The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT-PCR (reverse transcription-PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.
真皮间充质干细胞（DMSCs）是多能干细胞，能够在体外分化为多种细胞类型。关于人类、小鼠、兔子及其他哺乳动物的真皮间充质干细胞，已经开展了大量研究工作，但尚未有关于牛真皮间充质干细胞的相关文献发表。在本研究中，我们从牛身上分离并建立了真皮间充质干细胞系，从而开启了对这些细胞的进一步研究，如生长动力学、特殊表面抗原检测以及用于鉴定细胞系生物学特性的逆转录-聚合酶链反应（RT-PCR）分析。此外，还在体外诱导真皮间充质干细胞分化为脂肪细胞、成骨细胞和神经细胞。我们的结果表明，从牛身上分离得到的真皮间充质干细胞与其他物种的真皮间充质干细胞具有相似的生物学特性。它们的多向分化能力预示着在组织工程和诱导多能干细胞方面可能的应用模式。
