[鞘内注射甲泼尼龙琥珀酸钠对急性脊髓损伤兔的影响]

[Effects of intrathecal injection of methylprednisolone sodium succinate in acute spinal cord injury rabbits].

作者信息

Wang Kai-feng, Liu Hai-ying, Wang Bo, Wang Hui-min, Qian Ya-long, Zhu Zhen-qi, Miao Ke-nan, Zhang Heng-wei

机构信息

Department of Spinal Surgery, Peking University People's Hospital, Beijing 100044, China.

出版信息

Zhonghua Wai Ke Za Zhi. 2013 May 1;51(5):426-31.

PMID:23958166

Abstract

OBJECTIVE

To investigate the effects and apoptosis of intrathecal injection of Methylprednisolone Sodium Succinate (MPss) for acute spinal cord injury (SCI) in New Zealand rabbits.

METHODS

Seventy-two healthy New Zealand rabbits were used for the procedure and were randomly divided into two groups: SCI group and SHAM group, which was both divided into 6 subgroups, such as the vehicle group, the MPss intrathecal injection groups (1.5 mg/kg, 3.0 mg/kg, 6.0 mg/kg group), the MPss intravenous injection group and the combined injection group. TARLOV score was tested daily to evaluate the motor function. The rabbits were sacrificed 7 days after the surgery and the thoracic spinal cord sections and the sacral sections where MPss was injected were harvested for HE and TUNEL staining. Two-Factors Repeated Measures analysis of variance for TARLOV scores tested at various times and One-Way ANOVA analysis of variance for data between groups were used.

RESULT

Seven days after surgery in SCI group, there was no statistical difference between the TARLOV scores of intrathecal injection of MPss 3.0 mg/kg group, 6.0 mg/kg group and MPss intravenous injection group (P > 0.05), which were all better than the vehicle group (F = 4.762, P < 0.05). Referring to the lymphocyte infiltration at the injury site in SCI group, there was statistical difference between MPss intrathecal injection 6.0 mg/kg group (1.33 ± 0.21) and the vehicle group (2.67 ± 0.21) (F = 5.793, P < 0.05) and no statistical difference between intrathecal injection of MPss 6.0 mg/kg group and MPss intravenous injection group (P > 0.05). As for the lymphocyte infiltration at the intrathecal injection site in SHAM group, there was statistical difference between MPss intrathecal injection 6.0 mg/kg group (2.50 ± 0.55) and the vehicle group (0.50 ± 0.55) (F = 17.333, P < 0.05). TUNEL staining in SCI group showed statistical difference between MPss intrathecal injection 6.0 mg/kg group (6.3 ± 1.5) and the vehicle group (20.3 ± 2.2) (F = 71.279, P < 0.05).

CONCLUSIONS

Intrathecal injection of MPss can improve the functional recovery of lower limb and decrease apoptosis of neuron cells,which can provide same effects as the traditional intravenous injection of MPss in New Zealand rabbits.

摘要

目的

探讨鞘内注射甲泼尼龙琥珀酸钠（MPss）对新西兰兔急性脊髓损伤（SCI）的影响及凋亡情况。

方法

72只健康新西兰兔用于该实验，随机分为两组：SCI组和假手术组（SHAM组），每组再分为6个亚组，如溶剂对照组、鞘内注射MPss组（1.5mg/kg、3.0mg/kg、6.0mg/kg组）、静脉注射MPss组和联合注射组。每天检测TARLOV评分以评估运动功能。术后7天处死兔子，取胸段脊髓节段及注射MPss的骶段脊髓节段进行HE和TUNEL染色。对不同时间点的TARLOV评分进行两因素重复测量方差分析，对组间数据进行单因素方差分析。

结果

SCI组术后7天，鞘内注射3.0mg/kg MPss组、6.0mg/kg MPss组及静脉注射MPss组的TARLOV评分之间无统计学差异（P>0.05），均优于溶剂对照组（F = 4.762，P<0.05）。在SCI组损伤部位淋巴细胞浸润方面，鞘内注射6.0mg/kg MPss组（1.33±0.21）与溶剂对照组（2.67±0.21）之间有统计学差异（F = 5.793，P<0.05），鞘内注射6.0mg/kg MPss组与静脉注射MPss组之间无统计学差异（P>0.05）。在SHAM组鞘内注射部位淋巴细胞浸润方面，鞘内注射6.0mg/kg MPss组（2.50±0.55）与溶剂对照组（0.50±0.55）之间有统计学差异（F = 17.333，P<0.05）。SCI组TUNEL染色显示，鞘内注射6.0mg/kg MPss组（6.3±1.5）与溶剂对照组（20.3±2.2）之间有统计学差异（F = 71.279，P<0.05）。