Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Departamento SAMP, FCV - UNCPBA, Campus Universitario, 7000 Tandil, Buenos Aires, Argentina.
Res Vet Sci. 2013 Dec;95(3):991-5. doi: 10.1016/j.rvsc.2013.07.018. Epub 2013 Aug 16.
Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele 1501 has been associated with high proviral load in peripheral blood while allele 0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB30902) or susceptibility (BoLA DRB31501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR® Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle.
先前的研究表明，BoLA DRB3 基因多态性与牛白血病病毒（BLV）感染谱之间存在显著关联。等位基因1501 的存在与外周血中高前病毒载量有关，而等位基因0902 则与低前病毒载量有关。本研究旨在开发等位基因特异性实时 PCR 方法，以鉴定与 BLV 进展相关的抗性（BoLA DRB30902）或敏感性（BoLA DRB31501）的牛。设计了特异性引物，并通过实时 PCR 进行差异扩增，并通过外周血 DNA 样本中的 SYBR® Green 染料进行监测。还调整了传统 PCR 扩增（终点扩增）的条件。这些方法快速、简单，适合高通量筛选，并可辅助 BLV 抗性和敏感性牛的标记辅助选择。
