Cordero-Pulido Rosa María, Martínez-Herrera David Itzcóatl, Vivanco-Cid Héctor, Villagómez-Cortés José Alfredo, Arendt Maja Louise, Grube-Pagola Peter, Domínguez-Alemán Carlos Alonso
Department of Veterinary Histology, Faculty of Veterinary Medicine and Animal Sciences, University of Veracruz, Veracruz, Mexico.
Department of Veterinary Microbiology, Faculty of Veterinary Medicine and Animal Sciences, University of Veracruz, Veracruz, Mexico.
Vet Res Forum. 2023;14(8):457-460. doi: 10.30466/vrf.2022.555834.3510. Epub 2023 Aug 15.
The objective of this study was to determine the prevalence of bovine leukosis virus (BLV) in specialized and dual-purpose dairy cows located in the central zone of Veracruz state in Mexico, using endpoint polymerase chain reaction (PCR). The study population consisted of 307 specialized dairy cows and 95 dual-purpose cows from 13 municipalities located in the study area. All cows were apparently healthy and ≥ 3 years old. Cows were stratified by age (3 - 5, 6 - 8 and ≥ 9 years). The overall prevalence of infection was 6.96%; the calculated prevalence in dairy cows was 7.82% and in dual-purpose cows it was 4.21%. The municipality with the highest proportion was Acajete (14.28%), followed by Huatusco and Tomatlán (11.53%). The association analysis confirms the infection's independence to the cows' productive purpose. The results by age strata were 3 - 5 (4.60%), 6 - 8 (8.00%) and ≥ 9 (18.40%) with X = 9.96, with an odds ratio of 4.68 for the stratum ≥ 9 years with a significant difference. The present study determined the prevalence of proviral DNA of BLV in dairy and dual-purpose cows in six municipalities in the central zone of Veracruz state, Mexico, using endpoint PCR.
本研究的目的是利用终点聚合酶链反应（PCR）测定位于墨西哥韦拉克鲁斯州中部地区的专门化奶牛和兼用型奶牛中牛白血病病毒（BLV）的流行情况。研究群体包括来自研究区域内13个市的307头专门化奶牛和95头兼用型奶牛。所有奶牛表面健康且年龄≥3岁。奶牛按年龄分层（3 - 5岁、6 - 8岁和≥9岁）。总体感染率为6.96%；计算得出专门化奶牛的感染率为7.82%，兼用型奶牛的感染率为4.21%。感染比例最高的市是阿卡杰特（14.28%），其次是瓦图斯克和托马特拉（11.53%）。关联分析证实感染与奶牛的生产用途无关。按年龄分层的结果为3 - 5岁（4.60%）、6 - 8岁（8.00%）和≥9岁（18.40%），X = 9.96，≥9岁年龄组的优势比为4.68，存在显著差异。本研究利用终点PCR测定了墨西哥韦拉克鲁斯州中部地区六个市的专门化奶牛和兼用型奶牛中BLV前病毒DNA的流行情况。
