Analytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:61-6. doi: 10.1016/j.colsurfb.2013.07.040. Epub 2013 Jul 29.
In this study, single-use pencil graphite electrodes (PGEs) were modified by gold nanorods (AuNRs) and then single stranded oligonucleotide probe molecules (ssODNs) were immobilized onto the surface of AuNRs modified PGE. The electrochemical characterizations using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were firstly performed to present the successfull modification of AuNR onto PGE surface. Under the optimum experimental conditions based on enhanced sensor response, single stranded thiol linked DNA probe ssODNs was immobilized onto AuNR modified PGE and accordingly, a sequence-selective DNA hybridization related to Hepatitis B virus (HBV) DNA was performed at the surface of AuNR modified electrodes. The impedimetric sequence-selective HBV DNA hybridization was then explored in the case of complementary (target), or noncomplementary (NC) DNA sequences.
在这项研究中，我们用金纳米棒（AuNRs）对一次性铅笔式石墨电极（PGEs）进行修饰，然后将单链寡核苷酸探针分子（ssODNs）固定在 AuNRs 修饰的 PGE 表面上。我们首先使用电化学阻抗谱（EIS）和循环伏安法（CV）技术对 AuNR 成功修饰到 PGE 表面进行了电化学表征。在基于增强传感器响应的最佳实验条件下，将硫醇连接的单链 DNA 探针 ssODNs 固定在 AuNR 修饰的 PGE 上，从而在 AuNR 修饰电极表面上进行了与乙型肝炎病毒（HBV）DNA 相关的序列选择性 DNA 杂交。然后，在互补（靶标）或非互补（NC）DNA 序列的情况下，探索了基于阻抗的序列选择性 HBV DNA 杂交。
