Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100, Izmir, Turkey.
Colloids Surf B Biointerfaces. 2012 Jun 15;95:222-8. doi: 10.1016/j.colsurfb.2012.02.042. Epub 2012 Mar 7.
Single walled carbon nanotubes (SWCNT)-chitosan (CHIT) modified pencil graphite electrodes (PGEs) were developed for monitoring of DNA hybridization. SWCNT-chitosan modified PGE (CNT-CHIT-PGE), Chitosan modified PGE (CHIT-PGE) and unmodified PGE (bare-PGE) were firstly characterized by using scanning electron microscopy (SEM), and their electrochemical behaviors were investigated using electrochemical impedance spectroscopy (EIS). The concentrations of CHIT, carbon nanotube (CNT) and also amino linked DNA probe etc. were respectively optimized in order to obtain the better working conditions of CNT-CHIT modified PGE in DNA analysis. The sequence selective DNA hybridization related to Hepatitis B virus (HBV) was then explored in the case of hybridization between amino linked HBV probe and its complementary (target), or noncomplementary (NC), or mismatch (MM) sequences, and also hybridization in mixture sample.
单壁碳纳米管（SWCNT）-壳聚糖（CHIT）修饰的铅笔芯石墨电极（PGE）被开发用于监测 DNA 杂交。SWCNT-壳聚糖修饰的 PGE（CNT-CHIT-PGE）、壳聚糖修饰的 PGE（CHIT-PGE）和未修饰的 PGE（裸-PGE）首先通过扫描电子显微镜（SEM）进行了表征，并通过电化学阻抗谱（EIS）研究了它们的电化学行为。分别优化了壳聚糖（CHIT）、碳纳米管（CNT）以及氨基连接的 DNA 探针等的浓度，以获得 CNT-CHIT 修饰的 PGE 在 DNA 分析中的更佳工作条件。然后，在氨基连接的 HBV 探针与其互补（靶标）、非互补（NC）或错配（MM）序列之间的杂交，以及在混合样品中的杂交情况下，探讨了与乙型肝炎病毒（HBV）相关的序列选择性 DNA 杂交。
