Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100 Izmir, Turkey.
Talanta. 2010 Oct 15;82(5):1680-6. doi: 10.1016/j.talanta.2010.07.040. Epub 2010 Jul 24.
In this study, SnO(2) nanoparticles (SNPs)-poly(vinylferrocenium) (PVF(+)) modified single-use graphite electrodes were developed for electrochemical monitoring of DNA hybridization. The surfaces of polymer modified and polymer-SNP modified pencil graphite electrodes (PGEs) were firstly characterized by using SEM analysis. The electrochemical behaviours of these electrodes were also investigated using the differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. The polymer-SNP modified PGEs were then tested for the electrochemical sensing of DNA based on the changes at the guanine oxidation signals. Experimental parameters, such as; different modifications in DNA oligonucleotides, DNA probe concentrations were examined to obtain more sensitive and selective electrochemical signals for nucleic acid hybridization. After optimization studies, DNA hybridization was investigated in the case of complementary of hepatitis B virus (HBV) probe, mismatch (MM), and noncomplementary (NC) sequences.
在这项研究中，开发了 SnO(2) 纳米粒子 (SNP)-聚(二茂铁乙烯基)(PVF(+)) 修饰的一次性石墨电极，用于电化学监测 DNA 杂交。首先通过 SEM 分析对聚合物修饰和聚合物-SNP 修饰的铅笔石墨电极 (PGE) 的表面进行了表征。还使用差分脉冲伏安法 (DPV) 和电化学阻抗谱 (EIS) 技术研究了这些电极的电化学行为。然后，基于鸟嘌呤氧化信号的变化，对聚合物-SNP 修饰的 PGE 进行了基于 DNA 的电化学传感测试。检查了实验参数，例如 DNA 寡核苷酸的不同修饰、DNA 探针浓度，以获得更灵敏和选择性的用于核酸杂交的电化学信号。经过优化研究，研究了乙型肝炎病毒 (HBV) 探针互补、错配 (MM) 和非互补 (NC) 序列的 DNA 杂交情况。
