Johns Hopkins Asthma and Allergy Center, Baltimore, Md.
J Allergy Clin Immunol. 2013 Oct;132(4):777-87. doi: 10.1016/j.jaci.2013.06.038. Epub 2013 Aug 16.
Both the treatment of patients with allergic diseases and the study of allergic disease mechanisms depend on a wide variety of assays that in various ways assess the presence and function of IgE antibody. The study of allergic diseases could benefit from the study of its 2 principle cellular participants, mast cells and basophils, but the basophil is more accessible than mast cells for ex vivo studies. Its functionality is tested by using 2 predominant methodologies: the secretion of mediators of allergic inflammation and the expression of proteins on the plasma membrane after stimulation. Each approach has benefits. There are also many operational details to consider regardless of which general approach is taken, and proper interpretation of the methods requires a good understanding of the reagents used and the receptors expressed on basophils and a detailed understanding of the factors regulating aggregation of cell-surface IgE.
无论是治疗过敏性疾病患者，还是研究过敏性疾病机制，都依赖于各种各样的检测方法，这些方法通过不同的方式评估 IgE 抗体的存在和功能。对过敏性疾病的研究可以从研究其两个主要的细胞参与者，肥大细胞和嗜碱性粒细胞中获益，但嗜碱性粒细胞比肥大细胞更容易进行离体研究。通过使用两种主要的方法学来测试其功能：过敏炎症介质的分泌和刺激后质膜蛋白的表达。每种方法都有其优点。无论采用哪种一般方法，都有许多操作细节需要考虑，并且正确解释这些方法需要很好地了解用于检测的试剂以及嗜碱性粒细胞上表达的受体，以及详细了解调节细胞表面 IgE 聚集的因素。
