A.G错配寡核苷酸d(CGCAAGCTGGCG)的晶体结构和序列依赖性构象
Crystal structure and sequence-dependent conformation of the A.G mispaired oligonucleotide d(CGCAAGCTGGCG).
作者信息
Webster G D, Sanderson M R, Skelly J V, Neidle S, Swann P F, Li B F, Tickle I J
机构信息
Cancer Research Campaign Biomolecular Structure Unit, Institute of Cancer Research, Sutton, Surrey, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6693-7. doi: 10.1073/pnas.87.17.6693.
Abstract
The crystal structure of the dodecanucleotide d(CGCAAGCTGGCG) has been determined to a resolution of 2.5 A and refined to an R factor of 19.3% for 1710 reflections. The sequence crystallizes as a B-type double helix, with two G(anti).A(syn) base pairs. These are stabilized by three-center hydrogen bonds to pyrimidines that induce perturbations in base-pair geometry. The central AGCT region of the helix has a wide (greater than 6 A) minor groove.
摘要
已确定十二聚体d（CGCAAGCTGGCG）的晶体结构，分辨率为2.5埃，并针对1710个反射将其精修至R因子为19.3%。该序列以B型双螺旋形式结晶，含有两个G（反式）.A（顺式）碱基对。它们通过与嘧啶的三中心氢键得以稳定，这会引起碱基对几何结构的扰动。螺旋的中央AGCT区域具有较宽（大于6埃）的小沟。
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