通过质子磁共振光谱法检测脱氧核糖核苷酸中的鸟嘌呤X腺嘌呤碱基对。
Detection of a guanine X adenine base pair in a decadeoxyribonucleotide by proton magnetic resonance spectroscopy.
作者信息
Kan L S, Chandrasegaran S, Pulford S M, Miller P S
出版信息
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4263-5. doi: 10.1073/pnas.80.14.4263.
Abstract
A decadeoxyribonucleotide, d(C-C-A-A-G-A-T-T-G-G) (I), forms a duplex in solution. The base pairing pattern in this duplex was studied by proton nuclear magnetic resonance spectroscopy. Five NH...N hydrogen-bonded proton resonances were observed, and they were assigned by nuclear Overhauser enhancement experiments as well as by comparison to five previously assigned NH...N hydrogen-bonded proton resonances in a self-complementary duplex of similar sequence, d(C-C-A-A-G-C-T-T-G-G) (II). The results suggest that the central -G-A- residues of I form G X A base pairs in the helical state. The fact that the H2 proton of A at the sixth position from the 5' end of I showed nuclear Overhauser enhancement when the NH...N hydrogen-bonded proton resonance of G X A was irradiated suggests that the bases of the G X A base pair are oriented in an anti-anti conformation. Comparison of the linewidths at the half height of the NH...N hydrogen-bonded proton resonances of I at 1 degree C suggest that the G X A base pairs are less stable than adjacent A X T base pairs.
摘要
十脱氧核糖核苷酸d（C-C-A-A-G-A-T-T-G-G）（I）在溶液中形成双链体。通过质子核磁共振光谱研究了该双链体中的碱基配对模式。观察到五个NH...N氢键质子共振，并通过核Overhauser增强实验以及与序列相似的自互补双链体d（C-C-A-A-G-C-T-T-G-G）（II）中五个先前确定的NH...N氢键质子共振进行比较来进行归属。结果表明，I的中心-G-A-残基在螺旋状态下形成G×A碱基对。当照射G×A的NH...N氢键质子共振时，I的5'端第六位的A的H2质子显示出核Overhauser增强，这一事实表明G×A碱基对的碱基呈反-反构象取向。在1℃下I的NH...N氢键质子共振半高宽的比较表明，G×A碱基对比相邻的A×T碱基对稳定性更低。
相似文献
1
Detection of a guanine X adenine base pair in a decadeoxyribonucleotide by proton magnetic resonance spectroscopy.通过质子磁共振光谱法检测脱氧核糖核苷酸中的鸟嘌呤X腺嘌呤碱基对。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4263-5. doi: 10.1073/pnas.80.14.4263.
2
Base pair mismatches and carcinogen-modified bases in DNA: an NMR study of G.T and G.O4meT pairing in dodecanucleotide duplexes.DNA中的碱基对错配和致癌物修饰碱基：十二核苷酸双链体中G.T和G.O4meT配对的核磁共振研究
Biochemistry. 1988 Jan 12;27(1):108-15. doi: 10.1021/bi00401a018.
3
Deoxyguanosine-deoxyadenosine pairing in the d(C-G-A-G-A-A-T-T-C-G-C-G) duplex: conformation and dynamics at and adjacent to the dG X dA mismatch site.d(C-G-A-G-A-A-T-T-C-G-C-G)双链体中脱氧鸟苷-脱氧腺苷配对：dG×dA错配位点及其相邻位点的构象与动力学
Biochemistry. 1984 Jul 3;23(14):3207-17. doi: 10.1021/bi00309a015.
4
NMR and molecular modeling evidence for a G.A mismatch base pair in a purine-rich DNA duplex.富含嘌呤的DNA双链中G·A错配碱基对的核磁共振和分子模拟证据
Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):26-30. doi: 10.1073/pnas.88.1.26.
5
6
Structural studies of the O6meG.T interaction in the d(C-G-T-G-A-A-T-T-C-O6meG-C-G) duplex.d（C-G-T-G-A-A-T-T-C-O6meG-C-G）双链体中O6meG.T相互作用的结构研究
Biochemistry. 1986 Mar 11;25(5):1036-42. doi: 10.1021/bi00353a013.
7
Conformation and interaction of short nucleic acid double-stranded helices. II. Proton magnetic resonance studies on the hydrogen-bonded NH-N protons of ribosyl ApApGpCpUpU helix.短核酸双链螺旋的构象与相互作用。II. 核糖基ApApGpCpUpU螺旋中氢键连接的NH-N质子的质子磁共振研究。
Biochemistry. 1975 Nov 4;14(22):4864-9. doi: 10.1021/bi00693a013.
8
Sequence dependence of base-pair stacking in right-handed DNA in solution: proton nuclear Overhauser effect NMR measurements.溶液中右手螺旋DNA碱基对堆积的序列依赖性：质子核Overhauser效应核磁共振测量。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3908-12. doi: 10.1073/pnas.80.13.3908.
9
A study of secondary and tertiary solution structure of yeast tRNA(Asp) by nuclear magnetic resonance. Assignment of G.U ring NH and hydrogen-bonded base pair proton resonances.通过核磁共振研究酵母天冬氨酸转运核糖核酸（tRNA(Asp)）的二级和三级溶液结构。G.U环NH和氢键碱基对质子共振的归属。
Biochemistry. 1976 May 4;15(9):1883-8. doi: 10.1021/bi00654a014.
10
NMR studies of the stable mismatch purine-thymine in the self-complementary d(CGPuAATTTCG) duplex in solution.溶液中自互补双链体d(CGPuAATTTCG) 中稳定错配嘌呤-胸腺嘧啶的核磁共振研究。
Biochemistry. 1987 Sep 8;26(18):5646-50. doi: 10.1021/bi00392a010.
引用本文的文献
1
Structure of native four-repeat satellite III sequence with non-canonical base interactions.天然四重复卫星 III 序列的结构具有非规范碱基相互作用。
Nucleic Acids Res. 2024 Apr 12;52(6):3390-3405. doi: 10.1093/nar/gkae113.
2
Staggered intercalation of DNA duplexes with base-pair modulation by two distinct drug molecules induces asymmetric backbone twisting and structure polymorphism.两个不同药物分子通过碱基对调节使 DNA 双链交错排列，诱导不对称的骨架扭曲和结构多态性。
Nucleic Acids Res. 2022 Aug 26;50(15):8867-8881. doi: 10.1093/nar/gkac629.
3
Sequence-dependent structural changes in a self-assembling DNA oligonucleotide.一种自组装DNA寡核苷酸中依赖序列的结构变化
Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2471-8. doi: 10.1107/S1399004715019598. Epub 2015 Nov 26.
4
Structures of (5'S)-8,5'-Cyclo-2'-deoxyguanosine Mismatched with dA or dT.（5'S）-8,5'-环-2'-脱氧鸟苷与 dA 或 dT 错配的结构。
Chem Res Toxicol. 2012 Feb 20;25(2):478-90. doi: 10.1021/tx2005053. Epub 2012 Feb 6.
5
Formation of purine-purine mispairs by Sulfolobus solfataricus DNA polymerase IV.嗜热栖热菌DNA聚合酶IV形成嘌呤-嘌呤错配。
Biochemistry. 2008 Aug 5;47(31):8157-64. doi: 10.1021/bi800820m. Epub 2008 Jul 11.
6
Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells.氧化性DNA损伤的遗传效应：猴肾细胞中咪唑环开环的甲酰胺基嘧啶（Fapy损伤）和8-氧代嘌呤的比较诱变
Nucleic Acids Res. 2006 May 5;34(8):2305-15. doi: 10.1093/nar/gkl099. Print 2006.
7
Spectroscopic characterization of interstrand carbinolamine cross-links formed in the 5'-CpG-3' sequence by the acrolein-derived gamma-OH-1,N2-propano-2'-deoxyguanosine DNA adduct.由丙烯醛衍生的γ-羟基-1,N2-丙基-2'-脱氧鸟苷DNA加合物在5'-CpG-3'序列中形成的链间甲醇胺交联的光谱表征。
J Am Chem Soc. 2005 Dec 21;127(50):17686-96. doi: 10.1021/ja053897e.
8
Cleavage of fragments containing DNA mismatches by enzymic and chemical probes.通过酶学和化学探针切割含有DNA错配的片段。
Biochem J. 2003 May 1;371(Pt 3):697-708. doi: 10.1042/BJ20021847.
9
Through-bond correlation of adenine H2 and H8 protons in unlabeled DNA fragments by HMBC spectroscopy.通过HMBC光谱法对未标记DNA片段中腺嘌呤H2和H8质子进行键间相关分析。
J Biomol NMR. 1996 Sep;8(2):207-12. doi: 10.1007/BF00211166.
10
The thermal stability of DNA fragments with tandem mismatches at a d(CXYG).d(CY'X'G) site.在d(CXYG).d(CY'X'G)位点具有串联错配的DNA片段的热稳定性。
Nucleic Acids Res. 1996 Feb 15;24(4):707-12. doi: 10.1093/nar/24.4.707.
本文引用的文献
1
STRUCTURE OF A RIBONUCLEIC ACID.核糖核酸的结构
Science. 1965 Mar 19;147(3664):1462-5. doi: 10.1126/science.147.3664.1462.
2
Synthesis and template properties of an ethyl phosphotriester modified decadeoxyribonucleotide.一种乙基磷酸三酯修饰的十脱氧核糖核苷酸的合成及模板性质
Biochemistry. 1982 Oct 26;21(22):5468-74. doi: 10.1021/bi00265a014.
3
Proton nuclear magnetic resonance study of a self-complementary decadeoxyribonucleotide, C-C-A-A-G-C-T-T-G-G.一种自互补十脱氧核糖核苷酸C-C-A-A-G-C-T-T-G-G的质子核磁共振研究
Biochemistry. 1982 Dec 21;21(26):6723-32. doi: 10.1021/bi00269a017.
4
Structure, dynamics, and energetics of deoxyguanosine . thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution.溶液中自互补双链体d(CGTGAATTCGCG) 中脱氧鸟苷-胸苷摆动碱基对形成的结构、动力学和能量学
Biochemistry. 1982 Feb 2;21(3):437-44. doi: 10.1021/bi00532a003.
5
Adenine-guanine base pairing ribosomal RNA.腺嘌呤 - 鸟嘌呤碱基配对核糖体RNA
Nucleic Acids Res. 1982 Apr 24;10(8):2701-8. doi: 10.1093/nar/10.8.2701.
6
Nuclear magnetic resonance and nuclear Overhauser effect study of yeast phenylalanine transfer ribonucleic acid imino protons.酵母苯丙氨酸转移核糖核酸亚氨基质子的核磁共振与核Overhauser效应研究
Biochemistry. 1981 Mar 3;20(5):1147-56. doi: 10.1021/bi00508a016.
7
Nuclear Overhauser assignment of the imino protons of the acceptor helix and the ribothymidine helix in the nuclear magnetic resonance spectrum of Escherichia coli isoleucine transfer ribonucleic acid: evidence for costacked helices in solution.大肠杆菌异亮氨酸转移核糖核酸核磁共振谱中受体螺旋和核糖胸苷螺旋亚氨基质子的核Overhauser归属：溶液中堆积螺旋的证据
Biochemistry. 1982 Oct 12;21(21):5129-35. doi: 10.1021/bi00264a004.
8
Direct assignment of the dihydrouridine-helix imino proton resonances in transfer ribonucleic acid nuclear magnetic resonance spectra by means of the nuclear Overhauser effect.借助核Overhauser效应直接确定转移核糖核酸核磁共振谱中二氢尿嘧啶-螺旋亚氨基质子的共振峰
Biochemistry. 1982 Apr 13;21(8):1835-42. doi: 10.1021/bi00537a020.
9
Nuclear Overhauser effect study and assignment of D stem and reverse-Hoogsteen base pair proton resonances in yeast tRNAAsp.酵母天冬氨酸转运核糖核酸中D茎和反向-霍格施泰因碱基对质子共振的核Overhauser效应研究与归属
Nucleic Acids Res. 1981 Dec 21;9(24):7073-83. doi: 10.1093/nar/9.24.7073.
10
Structural and energetic consequences of noncomplementary base oppositions in nucleic acid helices.核酸螺旋中非互补碱基配对的结构和能量后果。
Prog Nucleic Acid Res Mol Biol. 1975;15(0):185-218. doi: 10.1016/s0079-6603(08)60120-8.
Zotero 插件